基因克隆的技术.docVIP

PAGE \* MERGEFORMAT 1 基因克隆技术 摘要：基因克隆技术是分子生物学的核心技术，其目的是获得某一基因或DNA片段的大量拷贝，用于深入分析基因的结构与功能，并可达到人为改造细胞以及物种遗传性状的目的。本论文主要从以下几个方面来介绍基因克隆技术：目的基因的获得 、目的基因和载体的连接、重组分子的扩增和鉴定。 关键词：目的基因；限制性内切酶；克隆；重组分子 ABSTRACT：Gene cloning technology is the core of molecular biology technology, its purpose is obtain a gene or DNA fragments of the copy, used for in-depth analysis the structure and function of genes, and may achieve human cells and the transformation of the species genetics purpose.This thesis mainly from the following several aspects to introduce gene cloning technology: the purpose of the gene for the pu