在高于米氏常数的底物浓度下用积分法测定尿酸酶活性.docVIP

　　在高于米氏常数的底物浓度下用积分法测定尿酸酶活性 作者：廖飞,朱小云,王咏梅,曾昭淳,左渝萍 【关键词】 尿酸酶 　　【Abstract】 AIM: To test the reliability of the integrated method for the assay of enzyme activity at substrate concentration above the MichaelisMenten constant (Km). METHODS: Uricase reaction curve was monitored by absorbance at 293 nm, and it was fit to the integrated MichaelisMenten rate equation with the predictor variable of reaction time for irreversible, single substrate reaction with the background absorbance as a nonlinear parameter while Km as a preset constant. RESULTS: Vm was sensitive to residual substrate concentrati