基因表达检测---RT-PCR3.pptVIP

操作步骤（一） RNA Preparation Prepare the plant total RNA by TRIzol Reagent. Dilute the Total RNA to the final concentration of 1 ?g / ?l by DU640. . Combine the following in a 0.5 ml sterile eppendorf tube: Total RNA 3?l (2-5?g) RNase-free DNase ? １?l (１u / ?l) 10× DNase ? buffer 1 ?l add DEPC-treated ddH2O 5 ?l Incubate at 37℃ for 15 min, then 70℃ for 10 min. 操作步骤（二）Reverse Transcriptase Reaction Add 1 ?l 500 ?g / ml oligo (dT) 15 primer, mix the contents of the tube by gently vortexing and collect the reaction by brief centrifugation.