高铝暴露对大鼠空腹血糖影响.docVIP

高铝暴露对大鼠空腹血糖影响 　　【摘要】目的通过观察Wistar大鼠在高铝暴露环境下的空腹血糖（Fasting blood glucose，FBG）变化情况，探讨高铝暴露对大鼠糖代谢的影响。方法将Wistar大鼠60只分为两组，高铝暴露组造模，对照组则以生理盐水尾静脉注射，同步实验，7天后于空腹抽取大鼠静脉血，使用己糖激酶法测定血糖，比较两组的FBG的变化。结果实验前对照组与高铝暴露组FBG水平比较差异无统计学意义（P0.05）；与实验前比较，实验后对照组FBG水平变化不大（P0.05），而高铝暴露组FBG水平则明显升高（P0.001）；实验后高铝暴露组FBG水平亦明显高于对照组（P0.001）。结论高铝暴露可导致大鼠血糖升高，引发糖代谢异常。 　　【关键词】高铝暴露；空腹血糖；糖代谢 　　中图分类号：R589.1文献标识码：ADOI：10.3969/j.issn.1003-1383.2017.03.004 　　【Abstract】ObjectiveTo investigate the effects of high aluminum exposure on glucose metabolism in Wistar rats by observing the changes of fasting blood glucose （FBG） in Wistar rats exposed to high aluminum.Methods60 Wistar rats were divided into two groups，high aluminum exposure group were used to establish rats model，and control group were injected with saline at caudal vein.Synchronous experiment were carried out between the two groups.7 days later，venous blood were extracted from rats，blood glucose was measured by hexokinase method，and the changes of FBG between the two groups were compared.ResultsBefore experiment，difference of FBG levels between the control group and the high aluminum exposure group was not statistically significant（P0.05）.Compared with that before experiment，the FBG level of the control group had no significant change after the experiment（P0.05），but that of the high aluminum exposure group significantly improved（P0.001），and that of the high aluminum exposure group was significantly higher than that of the control group（P0.001）.ConclusionHigh aluminum exposure can cause increase of blood glucose，which leads to abnormal glucose metabolism in rats. 　　【Key words】high aluminum exposure；FBG；carbohydrate metabolism 　　铝在大自然中蕴藏丰富，现代社会对铝的需求量也在不断增加，大量的人群从事与铝相关的工作。人体对铝的吸收主要通过三个途径：生活性摄入（食物）、医源性摄入（药物）、职业性摄入（接触）。其中，职业性铝接触对从事铝业工人的身体健康有着巨大的威胁[1]，同时还包括了大量周边与其接触的普通人群。而国外的研究有着相同的结果，Obukhova Tiu等对396例铝厂工人定期体检结果进行分析，发现铝产业工人2型糖尿病的发病率较正常人群升高，并且时间提前，并建议有必要对铝产业工人实施保护措施[2]。百色地区位处铝工业地区，周边有多个大型铝矿企业，人们从不同途径摄取铝的机会较正常人群增多，因此研究在高铝暴露环境下对血糖水平的影响十分有必要。 　　1对象与方法 　　1.1