复方黄连注射剂脱色工艺研究-天津中草药杂志社.PDF

·2386 · 中草药 Chinese Traditional and Herbal Drugs 第 44 卷 第 17 期 2013 年 9 月 复方黄连注射剂脱色工艺研究 1 1 2 1 3* 徐 芳 ，谭榀新 ，贺 兰 ，毛 宇 ，贺建华 1. 湖南农业大学食品科技学院，湖南 长沙 410128 2. 益阳市赫山区沧水铺人民医院，湖南 益阳 413064 3. 湖南农业大学动物科技学院，湖南 长沙 410128 摘 要：目的 确定制备复方黄连注射剂的最佳脱色工艺方法和条件。方法 以复方黄连注射剂未脱色药液为原料，以脱色 率、工艺成本、脱色后药液的气味变化及对耐药金黄色葡萄球菌的最低抑菌浓度（MIC ）等为评价指标，对活性炭吸附法、 铅盐沉法、氧化铝吸附法、石灰乳沉淀法、硅胶吸附法 5 种方法进行综合评价。结果 经过综合分析，确定最佳脱色方法为 硅胶吸附法。通过正交试验，确立硅胶吸附法的最佳工艺条件为硅胶用量 35%，温度40 ℃，时间 30 min，在该条件下脱色 率为 35.98%，复方黄连注射剂对耐药金黄色葡萄球菌的MIC 值为 7.8 mg/mL 。结论 用正交设计确定的硅胶吸附法最适脱 色条件可实现复方黄连注射剂的有效脱色。 关键词：复方黄连注射剂；正交试验；脱色工艺；最低抑菌浓度；硅胶吸附法；脱色率 中图分类号：R284.2 文献标志码：A 文章编号：0253 - 2670(2013)17 - 2386 - 05 DOI: 10.7501/j.issn.0253-2670.2013.17.009 Study on decolorization process of Compound Huanglian Injection 1 1 2 1 3 XU Fang , TAN Pin-xin , HE Lan , MAO Yu , HE Jian-hua 1. College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China 2. Cangshuipu Hospital of Heshan District of Yiyang, Yiyang 413064, China 3. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China Abstract: Objective To prepare the Compound Huanglian Injection (CHI) and to optimize the decolorization process of CHI. Methods The comprehensive effect of five decolorization processes (active carbon adsorption, lead salt precipitation method, aluminum oxide adsorption method, lime milk precipitation, and silica gel adsorption) was evaluated by the decolorization rate, cost of the process, chemical odor after bleaching, and minimuminhibitory concentration (MIC) to Staphylococcus aureus. Results The optimal method was silica gel adsorption method, and the optimum conditions calculated by orthogonal test were as fol