冷冻胁迫转金柑MLP2–1基因拟南芥的转录组测序和代谢通路.PDFVIP

() 2018, 44(4):376–381. DOI:10.13331/ki.jhau.2018.04.007 Journal of Hunan Agricultural University (Natural Sciences) 冷冻胁迫转金柑 MLP2–1 基因拟南芥的 转录组测序和代谢通路 1 1 2 1 1 1,3* (1. 410128 2. 410208 3. 410128) 摘 要(Fortunella margarita (Lour.) Swingle)MLP2–1 (Col–1)MLP2–1 MLP2–1 MLP2–1 1 422 551 871 GO 1 208 KEGG 474 COG 603 KEGG 关 键 词MLP2–1 中图分类号Q786 文献标志码A 文章编号1007− 1032(2018)04−0376−06 Transcriptome sequencing and metabolic pathway analysis of transgenic MLP2–1 gene in Arabidopsis under cold stress 1 1 2 1 1 1,3* TENG Jinjing , LI Mengyun , GUO Chun , HE Ying , QIAO Kewei , YANG Hua (1.College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan 410128,China; 2.First Affiliated Hospital, Hunan University of Chinese Medicine, Changsha, Hunan 410208,China; 3.China Crop Germplasm Innovation and Resource Utilization, Changsha, Hunan 410128,China) Abstract: In this study, miraculin–like protein 2(MLP2–1) gene of kumquat was cloned and transformed into wild–type Arabidopsis (Col–1) by Agrobacterium–mediated infiltration. The transcriptional data of transgenic Arabidopsis under colds tress was analyzed, which revealed the mechanism of MLP2–1 gene in Arabidopsis. The results showed that transgenic Arabidopsis had higher resistance than wild–type Arabidopsis under cold stress; comparing with the wild–type Arabidopsis , transcriptome analysis showed that 551 genes were up–regulated and 871 genes were down–regulated in total 1 422 differently expressed genes. Among of the