拟南芥RBCS-1A基因受光调节表达模式及其启动-中国科学技术协会.PDFVIP

作物学报 ACTA AGRONOMICA SINICA 2012, 38(9): 15611569 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2012.01561 拟南芥RBCS-1A 基因受光调节表达模式及其启动子遗传转化应用评 价 习雨琳1,2 周 朋1 宋梅芳1 李志勇1,3 孟凡华1 杨建平1,4,* 1 中国农业科学院作物科学研究所, 北京 100081; 2 中国农业科学院研究生院, 北京 100081; 3 河南农业大学农学院, 河南郑州 450002; 4 重庆邮电大学生物信息学院, 重庆 400065 : RBCS 1,5-/, PCR RBCS-1A , , AtRBCS-1A , ; , ; PCR 1 691 bp AtRBCS-1A , GUS , GUS , , AtRBCS-1A , AtRBCS-1A , : ; RBCS-1A ; ; ; Expression Patterns of Arabidopsis RBCS-1A Gene in Response to Light Treat- ments and Application Evaluation of Its Promoter in Transgenic Engineering 1,2 1 1 1,3 1 1,4,* XI Yu-Lin , ZHOU Peng , SONG Mei-Fang , LI Zhi-Yong , MENG Fan-Hua , and YANG Jian-Ping 1 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 Graduate School of Chinese Academy of Agricul- tural Sciences, Beijing 100081, China; 3 Agronomy College, Henan Agricultural University, Zhengzhou 450002, China; 4 College of Bio-information, Chongqing University of Posts and Tele-communication, Chongqing 400065, China Abstract: RBCS gene encodes the small subunit of Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), which catalyzes the rate-limiting step of CO2 fixation in photosynthesis. In this study, we analyzed