光照和pH对忍冬纤孔菌液体培养的影响-微生物学报
菌物学报
jwxt@ 15 March 2014, 33(2): 469‐476
Http:// Mycosystema ISSN1672‐6472 CN11‐5180/Q © 2014 IMCAS, all rights reserved.
简报 Short communication DOI: 10.13346/j.mycosystema.140008
光照和pH 对忍冬纤孔菌液体培养的影响
1 1 1 1 2 1*
余海尤 陈佳佳 田雪梅 崔宝凯 刘鸿高 戴玉成
1 北京林业大学微生物研究所 北京 100083
2 云南农业大学农学与生物技术学院 云南 昆明 650201
摘 要:以液体培养为基础,研究不同光照和培养液初始pH 条件下,忍冬纤孔菌培养过程中生物量、胞内外多酚含量,
以及胞内外提取物抗氧化活性的变化。结果显示,24h 光照轻微抑制菌丝体的生长,但能促进胞内外多酚的形成和提取
物对DPPH 自由基的抑制;在初始培养液pH5.3 条件下,菌丝体生物量最高,胞内外多酚含量和提取物活性最高。
关键词:药用菌,培养条件,生物量,DPPH 自由基清除活性
Effects of light and pH on the liquid culture of Inonotus lonicericola
1 1 1 1 2 1*
YU Hai‐You CHEN Jia‐Jia TIAN Xue‐Mei CUI Bao‐Kai LIU Hong‐Gao DAI Yu‐Cheng
1
Institute of Microbiology, Beijing Forestry University, Beijing 100083, China
2
College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, Yunnan 650201, China
Abstract : Under the different light and different initial pH of medium, the changes of biomass, polyphenolic content in
mycelia (intracellular polyphenol IP) and culture broth (extracellular polyphenol EP), and the variation of antioxidant
activities in liquid culture of Inonotus lonicericola were studied. The results showed that 24h light slightly inhibited the
growth of mycelia, but promoted the formation of IP and EP, and inhibition of DPPH radical. The initial culture medium of
pH5.3 was the best for the increase of my
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