临床医学毕业论文聚乙烯醇纺丝纤维编织在组织工程前交叉韧带支架材料中的应用.doc

X X 大学 毕业论文 聚乙烯醇纺丝纤维编织在组织工程前交叉韧带支架材料中的应用 姓 名：__________ 2014年6月25日 聚乙烯醇纺丝纤维编织在组织工程前交叉韧带支架材料中的应用 作者：戴丽冰，邹海燕，叶春婷，白利明，杨小红，沈雁，陈鸿辉，谭见容 【摘要】 探讨聚乙烯醇（PVA）纺丝纤维编织用I型胶原胶（COL-I）表面修饰后，构建组织工程前交叉韧带（ACL）支架材料的可行性。用PVA纺丝编织成条束状支架材料，用NIH-3T3细胞株和人前交叉韧带（HACL）细胞分别种植到PVA和经COL-I修饰过的PVA纺丝纤维编织（PVA/COL）支架材料上，立体培养。通过扫描电子显微镜对比评价材料经I型胶原胶表面修饰前后NIH-3T3细胞株和HACL细胞在纺丝编织材料上细胞附增殖情况；在电子拉力机上测试PVA纺丝纤维编织支架材料的力学性能。结果表明：NIH-3T3细胞株和HACL细胞在PVA和PVA/COL支架材料表面和孔隙内黏附增殖并分泌细胞外基质，在PVA/COL支架材料上细胞黏附数量明显增多； COL-I可促进NIH-3T3细胞株分泌细胞外基质，但对HACL细胞作用不明显。拉力测试该编织材料柔韧性强，最大负荷、极限应力和弹性模量分别为52.61 N、14.96 Mpa和202.08 Mpa。说明I型胶原可促进NIH-3T3细胞株和HACL细胞在聚乙烯醇纺丝纤维编织支架材料表面和孔隙内黏附、增殖，可促进NIH-3T3细胞株分泌细胞外基质。聚乙烯醇纺丝纤维编织材料具有一定的力学性能和细胞相容性，有望成为一种组织工程前交叉韧带支架材料。 【关键词】 聚乙烯醇；纺丝编织；I型胶原；前交叉韧带；组织工程 Abstract：To investigate the application feasibility of PVA scaffold modified by COL-I to construct anterior cruciate ligament tissue engineering.Braide stripe scaffold with PVA spinning collagen waving.NIH-3T3 cell line and human anterior cruciate ligament cells(HACL) were cultured and expanded in vitro and they were seeded into the scaffold which was braided with PVA, and PVA was modified by COL-I(PVA/COL). The growth situation of seeded cells into the scaffold was observed under the scanning electron microscope(SEM)，the mechanical properties of the scaffold was tested with the electronic tensioner.Results showed that the porous structure of the scaffold facilitated the attachment and proliferation of NIH-3T3 cell line an HACL cells, and extracellular matrix was secreted. Cells adhered and spreaded better on the PVA/COL scaffold than on the PVA materials. COL-I could promote NIH-3T3 cell line to secrete extracellular matrix ,but no obvious effect on HACL was found. Pull tests showed that the flexibility of the material was strong，the peak load was 52.61N，the ultimate stress and elastic modulus was 14.96 Mpa and 202.08 Mpa respectively.COL-I could promote the attachment and proliferation of NIH-3T3 cell line and HACL cells,it also can p