给水管网流速对水质与生物膜种群结构的影响.docxVIP

给水管网流速对水质和生物膜种群结构的影响收稿日期 收稿日期 Received: 接受日期 Accepted: *国家自然科学基金项目资助 Supported by the National Natural Science Foundation of China (No. **通讯作者 Corresponding author (E-mail: HYPERLINK mailto:yangwq@ lliu@) 黄保国1 顾 越1 陈国炜1 金菊良1 刘 丽1** 1合肥工业大学土木与水利工程学院 合肥 230009 摘 要 为了研究给水管网中主体水流速对水质和管壁生物膜种群的影响，采用给水管网模拟系统动态监测28日内的水质，对第28日的生物膜进行生物量的测定和16S rDNA测序。并利用统计分析方法解析主体水水质和生物膜之间的相互关系。结果表明：在流速为0.5 m/s时，主体水中TOC最为丰富（5.26 ± 0.17 mg/l），悬浮细菌总数最多（4.79 ± 0.02 log cell/ml），综合水质为最差（3.69），而且生物膜细菌总数最大（5.48 ± 0.06 log cell/cm2）；Pearson相关分析显示，不同流速下生物膜细菌总数与主体水水质指标中的电导率（R=0.73, p0.01），浊度（R=0.87, p0.001），TOC（R=0.94, p0.001）和细菌总数（R=0.92, p0.001）成正相关，与余氯（R=-0.68, p0.05）成负相关；在低流速和高流速条件下生物膜多样性较高，而在0.5 m/s流速下，生物膜细菌种群多样性处于最低水平；变形菌门在生物膜的群落结构中占主导地位。（图4 表4 参42） 关键词 流速；主体水水质；生物膜细菌；多样性指数 CLC Q93.333 Effect of water velocity on bulk water quality and pipe wall biofilm population in drinking water distribution systems* HUANG Baoguo1, GU Yue1, CHEN Guowei1, JIN Juliang1, LIU Li1** 1Schoo of Civil Engineering, Hefei University of Technology, Hefei 230009, China Abstract Objectives: In order to provide scientific basis and theoretical support for the optimal operation of water supply network, and to ensure the safety of water supply, this paper aims to study the effect of velocity on bulk water quality and wall biofilm. Methods: The bulk water quality was monitored within 28 days, biomass was measured and 16S rDNA was sequenced on the 28th day via a water distribution simulation system. The relationship between bulk water quality and biofilm was analyzed by statistical analysis. Results: The results showed that the flow velocity of 0.5 m/s yielded the most abundant TOC (5.26 ± 0.17 mg/l) in the bulk water, and the highest number of the bulk water bacteria(4.79 ± 0.02 log cell/ml), the worst bulk water quality, along with the highest number of biofilm bacteria(5.48 ± 0.06 log cell/cm2); Pearson correlation analysis showed that the total number of biofilm bacteria was positivel