（毕业学术论文设计）-有机废水中微生物絮凝剂产生菌的选育》.docVIP

PAGE 9 唐 山 学 院 毕 业 设 计 设计题目：有机废水中微生物絮凝剂产生菌的选育 系 别： 环境与化学工程系 班 级： 09石油（2）班 姓　 　名： 指 导 教 师： 2012年6月1 日 有机废水中微生物絮凝剂产生菌的选育 摘 要 用平板划线纯化法，从有机废水中分离出5支菌株，以各菌株发酵液对高岭土悬浮液絮凝效果为指标，筛选出1株微生物絮凝剂产生菌。经过形态学特征、生理生化反应初步鉴定该菌株为枯草芽孢杆菌。通过试验对其培养条件进行研究确定了其培养条件的优化组合，即培养时间24h、pH 7.3、温度30℃，并在该条件下培养后测其发酵液的絮凝率。结果显示絮凝率由优化之前的85%提高到了条件优化后的90.4%。 关键词：培养条件 絮凝效果 絮凝率 breeding of flocculant producing microorganism from organic wastewater Abstract Five strains were isolated from the sample of Organic wastewater collected from pond and artificial environment using the pure culture law. Through investigating the flocculating abilities for kaolin clay of culture broth.we found one strain,which had flocculating activity,was finally picked out among them.The strain was identified as Bacillu ssubtilus according to its morphological,physiological and biochemical characters. Then we did orthogonal experiment to study its culture conditions and determine the optimum culture conditions,which is culture time 1d, pH 7.5, temperature 30℃.Cultured in this condition and measured its rate of fermentation broth of flocculation.The result showed that the flocculation rate improved from 85% to 90.4% after optimization. Key words: Culture conditions; flocculation; flocculation rate 目 录 TOC \o 1-3 \h \z HYPERLINK \l _Toc326873096 1 引言 PAGEREF _Toc326873096 \h 1 HYPERLINK \l _Toc326873097 1.1 微生物絮凝剂 PAGEREF _Toc326873097 \h 1 HYPERLINK \l _Toc326873098 1.2 微生物絮凝剂的絮凝机理 PAGEREF _Toc326873098 \h 3 HYPERLINK \l _Toc326873099 1.3 微生物絮凝剂在水处理中的应用 PAGEREF _Toc326873099 \h 4 HYPERLINK \l _Toc326873100 2 试验 PAGEREF _Toc326873100 \h 9 HYPERLINK \l _Toc326873101 2.1试验所用仪器及材料 PAGEREF _Toc326873101 \h 9 HYPERLINK \l _Toc326873102 2.1.1 试验所用仪器 PAGEREF _Toc326873102 \h 9 HYPERLINK \l _Toc326873103 2.1.2 试验所用药品 PAGEREF _Toc326873103 \h 9 HYPERLINK \l _Toc326873104 2.2试验方法 PAGEREF _Toc326873104 \h 9 HYPERLIN