丹参素对高脂血症大鼠脂代谢调节机制的研究.docVIP

丹参素对高脂血症大鼠脂代谢调节机制的研究 　　[摘要]目的：探讨丹参素对高脂血症大鼠脂代谢的影响。方法：选用清洁级雄性SD大鼠60只，随机选取12只为基础对照组，饲以普通饲料，其余大鼠饲以高脂饲料，造模成功后随机分为高脂对照组和注射低、中、高（10，20，40mg?kg-1?d-1）剂量丹参素溶液（溶解于生理盐水）的试验组，2个对照组腹腔注射等体积生理盐水，每天腹腔注射1次，连续30d。检测血清TG，TC，HDL-C及LDL-C含量及肝脏ACC1，FAS，HMGR，CPT-I的mRNA表达水平。结果：注射丹参素后，大鼠血清中TG，TC，LDL-C含量降低，肝脏FAS和HMGR的mRNA表达水平显著降低。结论：丹参素通过降低TC含量，抑制LDL-C水平，及时清扫多余的胆固醇，有效调节高脂血症大鼠血脂水平;通过降低FASmRNA表达减少脂肪酸的合成，抑制HMGRmRNA表达来减少内源性胆固醇的合成。 　　[关键词]丹参素;高脂血症大鼠;血脂水平;mRNA表达水平 　　Study on regulatory effect of Danshensu on lipid metabolism of hyperlipidemia rats 　　CHEN Juanlt;supgt;1lt;/supgt;， DENG Junlt;supgt;2lt;/supgt;， ZHANG Yu-yanlt;supgt;1lt;/supgt;， LI Jian-guolt;supgt;1lt;/supgt;， WU Fei-yult;supgt;1lt;/supgt;， WAN Hai-tong1* 　　（1. College of Basic Medical Science， Zhejiang Chinese Medical University， Hangzhou 310053， China; 　　2. Safety Evaluation Research Center， Zhejiang Academy of Medical Sciences， Hangzhou 310013， China） 　　[Abstract]Objective： To explore the effect of Danshensu on the lipid metabolism of hyperlipidemic rats. Method： Sixty clean male SD rats were selected. Twelve of them were selected in the basic control group and fed with common foods， and the remaining rats were fed with the high-fat feeds. After the successful modeling， they were randomly divided into the high-fat control group and low dose （10 mg?kg-1?d-1）， medium dose （20 mg?kg-1?d-1） and high dose （40 mg?kg-1?d-1） Danshensu （dissolved in saline） groups. Both of the two groups were abdominally injected with the same volume of normal saline once a day for consecutively 30 days. The serum TG， TC， HDL-C and liver ACC1， FAS， HMGR， CPT-I mRNA expressions were detected. Result and Conclusion： Danshensu could inhibit the LDL-C level， timely clear redundant cholesterol and effectively regulate the lipid metablism of hyperlipidemic rats by reducing the TC content， decrease the fatty acid by reducing the FAS mRNA expression， and reduce the synthesis levels of endogenous cholesterol by inhibit the HMGR mRNA expression. 　　[Key word