应激大鼠幽门区一氧化氮和神经元型一氧化氮合酶与胃内胆汁反流的关系.docVIP

应激大鼠幽门区一氧化氮和神经元型一氧化氮合酶与胃内胆汁反流的关系 胃肠病学2006年第11卷第4期 应激大鼠幽门区一氧化氮和神经元型 一 氧化氮合酶与胃内胆汁反流的关系 刘兴栋罗和生刘艳李玲 武汉大学人民医院消化内科(430060) ? 207? 背景:幽门区一氧化氮(NO)与幽门括约肌舒张功能密切相关,但从形态学上探讨神经元型一氧化氮合酶(nNOS) 与胃内胆汁反流关系的研究却较少.目的:探讨应激大鼠幽门区NO和nNOS含量变化对胃内胆汁反流的影响.方 法:55只大鼠随机分为正常对照组(n=10),实验组(n=30)和免疫组化组(n=15),后两组以水浸束缚应激诱导应激性 溃疡模型.实验组于应激开始后2,4,5,6,8和10h各取5只大鼠,分别检测胃内胆汁酸浓度和胃黏膜溃疡指数 (UI.Guth评分),以生化试剂盒检测幽门区NO含量.免疫组化组于应激开始后0,4和6h各取5只大鼠,以免疫 组化SP法结合图像分析检测幽门区nNOS免疫反应阳性产物的平均光密度值.结果:应激性溃疡大鼠幽门区N0 含量于应激结束后lh达到峰值(Plt;O.01);应激结束后2h,胃内胆汁酸浓度达到峰值(0.01);胃黏膜UI于应激结 束后4h达到峰值(Plt;O.O1).免疫组化染色显示应激性溃疡大鼠胃内胆汁反流增加时,幽门区肌间神经丛nNOS免疫 反应阳性产物平均光密度值显着增高(Plt;O.01).结论:NO能促进应激大鼠胃内胆汁反流.NO和nNOS参与了幽门括约 肌舒张功能的调控 关键词一氧化氮;神经元型一氧化氮合酶;胆汁反流;应激 CorrelationsbetweenNitricOxide.NeuronNitricOxideSynthaseandGastricBileReflUXinStressedRats PylorusLIUXingdong,LUOHesheng,LIUYon,LILing.DepartmentofGastroenterology,RenminHospitalofWuhon University,Wuhon(430060) Background:Thepyloricareanitricoxide(NO)iscloselycorrelatedwiththerelaxationfunctionofpyloricsphincter, butfewmorphologicalstudieshavebeencarriedoutontherelationshipbetweenneuronnitricoxidesynthasefnNOS1and bilerefluxinthestomach.Aims:ToappraisetheeffectsofpyloricNOandnNOSonbilerefluxinstressedratsstomach. Methods:Fifty-fiveratswererandomlydividedintothreegroups:normalcontrolgroup(n=lO),experimentalgroup(n=30) andimmunohistochemicalstaininggroup(n--15).Stressulcermodelwasconstructedbywater—immersionrestraintinthe lattertwogroups.Intheexperimentalgroup,thegastricbileacid,ulcerindex(UI)andpyloricNOweremeasuredat6 timepoints:2,4,5,6,8and10hoursafterthebeginningofstressinfiveratsofeach.respectively.Inimmunohisto— chemicalstaininggroup,themeanopticaldensityofnNOS—positiveproductsatthepyloricsphincterwasstudiedbySP immunohistochemistryandimageanalysis0,4and6hoursafterstartingthestressinfiveratsofeach. Results:The pyloricNOreachedthepeak1hourafterstress(Plt;O.01),andthegastricbileacidandUIreachedthemaximum2and4 hoursafterstress,respectively(lt;0.(】1).Accompaniedbytheincreasedbilereflux,themeanopticaldensityo