分子生物学张海红9章.ppt

DNA damage and their repair Causes Repair Direct reversal of DNA damage Base excision repair Nucleotide excision repair Recombination (DSB) repairs Translesion DNA synthesis damage spontaneously alkylation, oxidation and radiation base analogs intercalating agents 诱变剂的种类 1.260紫外辐射：环丁二聚 2.电离辐射 3.加热:A-P位点 化学诱变剂 1.碱基类似物： 5-溴尿嘧啶 2.脱氨剂：腺嘌呤——次黄嘌呤 3.烷化剂：甲基磺酸乙脂(EMS) 4.嵌入剂：EB 物理诱变剂 Chapter 9 If DNA naturally contained uracil instead of thymine, the deamination of cytosine will create a natrual base uracil which the repair system will not easily recognize. Can 5-mC ? T lesion be repaired?