一株丁二酸高产菌株的筛选与选育-生物技术通报.PDF

生物技术通报 ·研究报告· BIOTECHNOLOGY BULLETIN ( ) 2015, 31 3 :127-134 一株丁二酸高产菌株的筛选与选育 王乐  宇光海  杨硕晔  王为为  惠明 （河南工业大学生物工程学院，郑州 450001） 摘 要 ： 旨在提高丁二酸发酵生产水平，通过初筛、复筛从土壤中分离得到了高产丁二酸菌株，发酵结束后利用高效液相 色谱法检测丁二酸的产率为34.45%。对菌株进行紫外诱变选育，结果表明，在底物葡萄糖的浓度为 100 g/L 时，丁二酸的产率提 高了46%，达到了50.30%，残糖低于10 g/L。经形态学特征、生理生化指标测定以及16S rDNA 序列分析等，鉴定该菌株为放线杆菌。 关键词 ： 丁二酸 ；高产菌株；筛选；诱变 ；放线杆菌 DOI ：10.13560/ki.biotech.bull.1985.2015.04.018 Screening and Breeding of a Strain for High Yield of Succinic Acid Wang Le Yu Guanghai Yang Shuoye Wang Weiwei Hui Ming （College of Biological Engineering ，Henan University of Technology ，Zhengzhou 450001 ） Abstract: It was to improve the production of succinic acid. After the primary and secondary screening, a strain with high-yield of succinic acid was isolated from the soil. The succinic acid production of 34.45% was determined by the high performance liquid chromatography （HPLC）after the fermentation. Later, the UV mutagenic breeding of strains has been performed, resulting in the succinic acid production of 50.30% which increased by 46% in the substrate of glucose concentration of 100 g/L with the residual sugar lower than 10 g/L. After the determinations of morphological characteristics, physiological and biochemical index and 16S rDNA sequence analysis, the strain was identified as actinobacillus. : succinic acid ；high-producing strain ；screen ；mutagenesis ； Key words Actinobacillus 点 ［6］ 近年来，丁二酸应用的领域不断拓展，国际市 。丁二酸的生物法生产主要有两种方式，葡萄 场的需求量也迅猛增加 ［1］ 糖经糖酵解转化为磷酸烯醇式丙酮酸后，一方面继