分子生物学杨洋第三章-dna的复制--基于dna复制原理的pcr技术.ppt

Denaturation (变性): The target DNA (template) is separated into two stands by heating to 95℃ Primer annealing (退火): The temperature is reduced to around 55℃ to allow the primers to anneal. Polymerization (elongation, extension) (延伸): The temperature is increased to 72℃ for optimal polymerization step which uses dNTPs and requires Mg2+. Taq polymerase ：isolated from the thermophilic bacterium Thermus aquaticus (嗜热菌）, 耐热DNA聚合酶，耐高温 It has no 3’ to 5’ proofreading exonuclease activity High-accuracy DNA polymerase is available commercially(高保真酶） Discovery of PCR technique 希望大家不仅仅知道“是什么”，而且也了解