基于AU-PDMS复合SERS基底的制备及其在副溶血性弧菌检测中的应用.PDF

中国科技论文在线 基于AU-PDMS 复合SERS 基底的制备及 其在副溶血性弧菌检测中的应用 沈默斐，段诺，吴世嘉，王周平** 5 （江南大学食品学院，江苏无锡 214122 ） 摘要：本文研究了一种基于表面增强拉曼散射（SERS）的食物病原体副溶血弧菌的检测方 法。使用了Apt-Au-PDMS 膜组成的纳米结构作为SERS 基底。将适配体固定在连接了AuNPs 的PDMS 膜上作为捕获结构。将适配体和拉曼信号分子4-MBA 修饰在AuNPs 上，作为SERS 感应探针，从而通过适配体与靶标的特异性结合构建了基于拉曼光谱检测的固态的三明治结 10 构。副溶血性弧菌的浓度可以通过测量4-MBA 的SERS 强度来量化。在最佳条件下，副溶 血性弧菌浓度在 1.2×102〜1.2×106 cfu•mL-1 范围内与信号强度呈线性相关，检出限为 12 cfu•mL-1。这种基于超灵敏的适体SERS 检测结构其将会是一个有良好前景，适用与许多方 面的传感应用。 关键词：食源性致病菌；聚二甲基硅氧烷；纳米金；食品安全 15 中图分类号：TS207.4 PDMS gold nanoparticle composite film as structure for aptamers-based detection of Vibrio parahaemolyticus by surface-enhanced Raman spectroscopy SHEN Mofei, DUAN Nuo, WU Shijia, WANG Zhouping 20 (School of Food Science and Technology, Jiangnan University, Wuxi 214122, China) Abstract : This paper investigated a detection method based surface-enhanced Raman scattering (SERS) of the food pathogen Vibrio parahaemolyticus. Nanostructures consisting of Apt-Au-PDMS film were used as SERS substrates. An aptamer was immobilized on the fuctional PDMS film which covered AuNPs to act as a capture structure. AuNPs were modified with the aptamer and Raman reporter 25 molecule 4-MBA to act as a SERS sensing probes that binds to the target the same way as the Apt-Au-PDMS film. A robust sandwich structure based on Raman spectroscopy is established. The concentration of V. parahaemolyticus can be quantified by measurement of the