分子生物学张海红chapter15.ppt

CHAPTER 15 The Genetic Code Deciphering Cell-free translation of artificial RNAs (Nirinberg,1961) The triplet binding assay (Nirinberg,1964) tRNA和氨基酸、核糖体及三联体的结合是特异的 上述结合的复合体大分子是不能通过硝酸纤维滤膜的微孔，而tRNA- 氨基酸的复合体是可以通过的。 poly(U) ---UUU--- polyphenylalanine poly(C) ---CCC--- polyproline poly(A) ---AAA--- polylysine poly(G) --- did not work because of the complex secondary structure Attempt to Decipher: 1954, George Gamow Three rules govern the genetic code Features: The code is degenerate Wobble in the anticodon The code is nearly universal Suppressor mutations can res