菲厌氧降解对细菌和古细菌群落的影响.pdfVIP

北京大学学报 ( 自然科学版), 第 47 卷 , 第 5 期 , 2011 年 9 月　 Acta Scientiarum Naturalium Universitatis Pekinensis, Vol. 47, No. 5 (Sept. 2011)　 菲厌氧降解对细菌和古细菌群落的影响 张书颖 谢曙光† 北京大学环境科学与工程学院 , 北京 100871; † 通信作者 , E-mail: xiesg@ 摘要 利用 TRFLP 技术研究了受垃圾渗滤液污染的地下沉积物中细菌和古细菌群落在菲厌氧降解前后的变 化。结果表明 : 细菌群落在生物降解过程中变化很大, 物种丰度及 Shannon-Weiner 指数分别由 15 和 2.39 增 加到 23 和 2.88; 古细菌群落在生物降解过程中变化较小, 物种丰度及 Shannon-Weiner 指数变化不大。 关键词 多环芳烃; 生物降解; 微生物群落 ; TRFLP 技术 中图分类号 X172 Impact of Anaerobic Phenanthrene Biodegradation on Bacterial and Archaeal Communities ZHANG Shuying, XIE Shuguang† College of Environmental Sciences and Engineering, Peking University, Beijing 100871; † Corresponding author, E-mail: xiesg@ Abstract Terminal restriction fragment length polymorphism (TRFLP) was used to investigate the change of bacterial and archaeal communities in leachate-contaminated aquifer in response to anaerobic phenanthrene biodegradation. Results show that a great change in bacterial community occurred with phenanthrene biodegradation. The ribotype and Shannon-Weiner index increase from 15 to 23, and 2.39 to 2.88 respectively. However, archaeal community only shows an insignificant change with phenanthrene biodegradation, and ribotype and Shannon-Weiner index vary slightly. Key words polycyclic aromatic hydrocarbons (PAHs); biodegradation; microbial community; terminal restriction fragment length polymorphism (TRFLP) 卫生填埋是目前及今后相当长的时期内我国城 性 , 严重影响生态环境和人类健康 , 已被我国和其 市生活垃圾处理的主要方式 , 由于我国卫生填埋技