气相与液相层析质谱仪.PDF

GC-MS/LC-MS 氣相層析質譜儀 / 液相層 析質譜儀 交通大學應用化學系 謝有容 教授 國立交通大學 應用化學系貴重儀器中心 1. GC-MS氣相層析質譜儀 鄭彥如( 教授) 2. LC-MS 液相層析質譜儀 ( 李耀坤教授) 3. NMR (500 MHz) 核磁共振儀(莊士卿教授) 4. NMR (600 MHz) 核磁共振儀(孫仲銘教授) GC-MS氣相層析質譜儀 GC 離子源 質譜分析 偵測設備 高真空幫浦 主真空槽 資料處理系統 GC及質譜控制 輔助幫浦 GC-MS氣相層析質譜儀 LC-MS 液相層析質譜儀 A mixture of compounds is separated by liquid chromatography, then identified by mass spectrometry. 5 LC-MS 液相層析質譜儀 Distinction Between Mass and Weight  Mass is an invariant measure of the amount of matter in an object.  Weight is the force of attraction between an object and its surroundings (ex. earth). w = mg (w is the weight of an object, m is its mass, and g is the acceleration due to gravity) Atomic Mass Units  The atomic mass unit (amu) is based on a relative scale in which the reference is the 12C carbon isotope, which is exactly 12 amu.  The amu is 1/12 of the mass of one neutral 12C atom. 1 amu =1/12 of 12C  12C: 98.90 % 12 13C: 1.10 % 13.003355 C 12.0107 What is Mass Spectrometry ?  Mass Spectrometry (MS) is an analytical spectroscopic tool primarily concerned with the separation of molecular (and atomic) species according to their mass. http://www-methods.ch.cam.ac.uk/meth/ms/theory/index.html actually according to their mass to charge ratio (m/z) - Inorganic mass spectrometry - Organic and