抑制性消减杂交筛选和克隆果蝇七氟烷敏感性相关基因.PDFVIP

2009 32 1 ·65 · · · 孙玉明　曹云飞　吴飞翔　俞卫锋 　　【】　　(SSH), 。　, , SSH 。　, 77 , 56 ;58 , 52 ; 4 。, , Y ;2 。　 , 2 , , , 。 【】　;;;;; Scr ee ni ng a nd c lo ni ng se v of l urane se nsit iv it y ge ne s of us ing suppr e ssi o n subt ra c t ive hy bri d iza t io n i n Dr os o phi la me la nogas t e r　SUN Yuming, CAO Yunfei, WU Feixiang, YU Weifeng.　Department of Anesthesi- ology, Eastern Hepatobiliary Surgery Hospital, Secon Military Me ical University, Shanghai 200438, China 　　【A bs t ra c t】　Obj ec t i ve 　To explore the molecular mechanisms of pharmacological actionof inhaled anes- theticsby screening and cloning the sevoflurane sensitivity genes in three different strains of Drosophila melano- gaster.Me t hods 　Suppressionsubtractive hybridization (SSH) as appliedto detect mRNA levels of sevoflurane sensitivity genes inthesevoflurane-sensitivestrain, thesevoflurane-resistant strainand the ildtypestrainof Dro- sophila melanogaster;totally 243 differential fragments (cDNA) ere identified by SSH.Re s ult s 　Compared to ild type strain, there ere 77 upregulated and 56 do nregulated cDNAs insevoflurane-sensitivestrain;58 upreg- ulated and 52 do nregulated cDNAs in sevoflurane-resistant strain;four cDNAs ere upregulated in sevoflurane- sensitive strain hile do nregulated insevoflurane-resistant strain.Allsevoflurane-sensitivecNDAs ere located in x-chromosome (Chr.2).C o nc l usi o n　Genotyping profiles are associated ith sevoflurane sensitivity inthe three different strainsof Drosophila melanogaster, hich may cast lights onthe pharmacological mechanisms of inhalation anesthetics.(Shan