甜玉米种子活力差异近等基因系植株生育后期源库的关系初探.docVIP

甜玉米种子活力差异近等基因系植株生育后期源库的关系初探 　　摘 要 为探明甜玉米种子活力差异近等基因系植株生育后期源库关系，进一步阐明甜玉米种子活力形成的生理基础，以2组种子活力差异的近等基因系材料hv1与lv1、hv2与lv2为试材，研究其生育后期叶“源”和籽粒“库”的生理生化特性。结果表明：种子活力高的近等基因系材料（hv1和hv2）籽粒的可溶性糖含量较低，而可溶性蛋白含量较高；吐丝后，种子活力高的近等基因系材料（hv1和hv2）叶片的可溶性糖含量和可溶性蛋白含量均较高。研究还表明：籽粒可溶性糖含量与叶片可溶性糖含量、光合生理特性（除叶绿素b含量外）呈一定的负相关关系，而籽粒可溶性蛋白含量与吐丝后30 d叶片可溶性蛋白含量、叶绿素a含量和总叶绿素含量均显著正相关。因此，适度较强的叶片光合生理，保证光合产物的高效积累，降低籽粒可溶性糖含量，提高籽粒可溶性蛋白含量，是提高甜玉米种子活力的生理基础。 　　关键词 甜玉米 ；种子活力 ；近等基因系 ；源库关系 　　中图分类号 S513 文献标识码 A Doi：10.12008/j.issn.1009-2196.2016.05.009 　　Abstract In order to investigate the source-sink relationship between near-isogenic lines of sweet corn based on seed vigor differences at late growth stages， and further clarified the physiological basis of sweet corn seed vigor. This paper analysis the photosynthetic physiological characteristics， soluble protein content and soluble sugar content of the leaves at silking stage with high seed vigor sweet corn lines such as hv1， hv2， and low seed vigor sweet corn line such as lv1，lv2 as materials. The results show that the high seed vigor sweet corn lines （hv1 and hv2） have lower soluble sugar content but higher soluble protein content in seeds. After silking， high seed vigor sweet corn lines （hv1 and hv2） have higher soluble sugar content and soluble protein content in leaf. Seed soluble sugar content， leaf soluble sugar content and photosynthetic physiological characteristics （in addition to the content of chlorophyll b） are negative correlated， soluble protein content in mature seed， leaf soluble protein content， the chlorophyll content and total chlorophyll content at 30 d after silking are positive correlated. Therefore， moderately strong photosynthetic physiological characteristics can ensure the efficient accumulation of photosynthetic product. It can also reduce the grain soluble sugar and improve the soluble protein content， and finally improve the sweet corn seed vigor. 　　Keywords sweet corn ； seed vigor ； near-isogenic lines ； source-sink relation 　　甜玉米作为新型果蔬