抗幽门螺旋杆菌感染治疗对儿童肠道菌群的影响分析.docVIP

抗幽门螺旋杆菌感染治疗对儿童肠道菌群的影响分析 ［ ］目的研宄抗幽门螺旋杆菌感染治疗对 儿童肠道菌群的影响分析。方法按标准选取2015 年1月?2016年10月在我院就诊患儿104例，所有 患者入院后13C呼气试验，其中59例患者经检测后确 定Hp感染阳性，45例患者Hp感染阴性，取所有患儿 新鲜粪便2.0g，用10倍稀?法分别稀释至10-1、10-3、 10-5、10-7进行厌氧和需氧培养，选择培养肠道中的 典型菌落，如肠球菌、肠杆菌、酵母菌、双歧杆菌、 乳杆产气荚膜梭类杆菌进行培养。培养检测 乳杆 产气荚膜梭 类杆菌进行培养。培养检测 后所有患者采取阿莫西林+克拉霉素+奥美拉哇联合用 药，治疗1周后，所有患者再次进行粪便菌群培养。结 果Hp阳性组和Hp阴性组组间肠道菌落数量及B/E 比较，差异无统计学意义（P0.05）。与治疗前比较， 较，差异有统计学意义（P Hp阳性患儿治疗后酵Buga Hp阳性患儿治疗后酵 Buga 及产气荚膜梭菌检出率比 ［关键词］幽门螺旋杆菌；抗感染治疗；儿童肠道 群；影响 ［ ］R725.7 ［ ］A ［ ］ 2095-0616 (2017) 06-49-04 ［Abstract］ Objective To study the impact of Helicobacter pylori infection therapy to intestinal flora of children. Methods 104 children cured in our hospital from January 2015 to October 2016 were standardly selected.All the patients were given 13C breath test after admission.There were 59 cases of positive Hp infection, and 45 cases of negative Hp infection.Taking fresh stool 2.0g，and they were diluted to 10-1，10-3，10-5，10-7 to carry on anaerobic and aerobic culture by 10 fold dilution method.Typical colonies in the intestine were selected to culture，cuch as Enterococcus faecalis， enterobacter, yeast, Bifidobacterium, Lactobacillus, Clostridium perfringens， Bacteroides.After culture test， all the patients were treated with combination of amoxicillin, clarithromycin and omeprazole.After 1 weeks of treatment，all the patients were followed up by fecal bacteria culture. Results There was no significant difference between Hp positive group and Hp negative group in intestinal colony number and B/E （P〉0.05） .Compared with before treatment, there was significant difference in the detection rate of yeast and Clostridium perfringens in Hp positive children （P [Key words] Helicobacter pylori； Anti infective therapy； Intestinal flora of children； Impact 幽门螺旋杆菌（helicobacter pylori, Hp）目前证 实是胃、十二指肠溃疡发病的主要原因，它不仅可以 在强酸性条件下存活，难以消灭，更可以引发胃癌［1］。 近年来研究表明，全球一半以上的人都患有Hp感染， 而在儿童中，Hp感染者的数量则是以每年7%的增长 率递增，且随着年龄的增长，感染者数量进一步增加 ［2］。Hp感染不仅导致儿童出现胃、十二指肠溃荡，更 容易导致患儿营养不良及贫血［3］，大大危害儿童的发 育及身体健康。目前，临床上一般采取三联