烟草钟器腐霉分子鉴定与致病力测定.docVIP

烟草钟器腐霉的分子鉴定与致病力测定-农学论文 烟草钟器腐霉的分子鉴定与致病力测定 边传红1，王伟杰2，赵世民3，康业斌1 （1河南科技大学林学院，河南洛阳471003；2洛阳市烟草公司嵩县支公司，河南嵩县471400；3河南省烟草公司洛阳市公司，河南洛阳471023） 摘要：从河南省嵩县送检的烟草茎黑腐病样本上分离获得1 株腐霉菌，为明确该菌的种类及对烟草的致病力，对所得菌株进行形态学鉴定、DNA序列分析和致病性测定。结果表明，菌株菌落呈放射状型，并逐渐变为花瓣型，菌丝无隔；孢子囊球形、梨形；藏卵器球形；雄器钟状或不规则；rDNA-ITS 序列测定分析，该菌序列与GenBank上登录号KC014615.1 和GU133597.1 等的序列同源性大于99%，鉴定为钟器腐霉(Pythium vexans de Bary)。对培育到10 叶期的‘中烟100’品种接种，7 天后该菌的病情指数为48，对照瓜果腐霉(P. aphanidermatum)的病情指数为62.7，表明其对‘中烟100’的致病力不及瓜果腐霉。 关键词 ：烟草；钟器腐霉；rDNA-ITS；致病性测定 中图分类号：S432.4 文献标志码：A 论文编号：2014-0479 Molecular Identification and Pathogenicity of Pythium vexans Isolated from Tobacco Bian Chuanhong1, Wang Weijie2, Zhao Shimin3, Kang Yebin1 (1College of Forestry, Henan University of Science and Technology, Luoyang 471003, Henan, China;2Songxian Branch Company of Luoyang Tobacco Company, Songxian 471400, Henan, China;3Luoyang Tobacco Company of Henan Tobacco Company, Luoyang 471023, Henan, China) Abstract:Pythium was isolated from tobacco stems back rot samples in Song County of Henan Province. Inorder to know its classification and pathogenicity to tobacco, the morphologic characters that based onmicroscopy technology were examined and recorded, the rDNA- ITS sequences were analyzed, thepathogenicity tests were conducted. The results showed that the colonies were actinomorphic and graduallygrow into petaliform; hyphas were without separate; sporangiums were spherical or pear-shaped; archegoniumswere spherical; antheridiums are bell-shaped or irregular. According to the results of the analysis of rDNAITSsequences, the sequence homology was more than 99% when it was compared with the sequence ofregistration number kc014615.1 and gu133597 in GenBank. Therefore, the pathogen was identified asPythiumvexans de Bary. Seedings in ten leaf stage of‘Zhongyan 100’tobacco variety were inoculated withP. vexansde Bary, and the disease symptoms and the disease indexes were surveyed and recorded in the 7th day, thed