大黄蒽醌致泻作用及其机理的初步研究..docVIP

大黄蒽醌致泻作用及其机理的初步研宄 】目的比较大黄结合蒽醌、游离蒽醌致泻作用 的强度并探讨其相关机制。方法通过灌服高浓度的奶粉溶液 以营造高营养性环境，在此基础上考察了大黄结合蒽醌及游 离蒽醌对小鼠的致泻作用；并运用析因设计分析了奶粉在这 些致泻作用中的作用，同时还考察了大黄结合蒽醌及其游离 蒽醌体外对胃蛋白酶活性的影响。结果大黄游离蒽醌500, 1 OOOmg /kg均可使小鼠胃内容物和小肠内容物显著增加；大 黄结合蒽醌500，100 Omg/kg也可显著增加小鼠小肠内容的 重量，但对小鼠胃内容重量的影响作用较弱；各药物组均可 显著增加小鼠各肠段内的蛋白质浓度。析因设计的实验结果 显示，无论是大黄游离蒽醌或是结合蒽醌均可显著增加正常 小鼠的胃、小肠及全胃肠道的内容物及蛋白质的含量，并可 显著降低小鼠小肠、结肠C1-浓度和升高小鼠结肠K+浓度； 奶粉本身也具有与大黄蒽醌类似的作用，与之合用可增强大 黄蒽醌的作用。体外实验结果显示，无论是大黄游离蒽醌或 是结合型蒽醌对胃蛋白酶的活性均无明显影响。结论大黄游 离蒽醌和结合蒽醌均具有显著的致泻作用；其机理可能是通 过刺激胃肠道分泌、增加胃肠道内蛋白质浓度而产生容积性 导泻。 关键词】大黄结合蒽醌大黄游离蒽醌胃小肠结肠 ThelnducingDiar rheaEffectsanditsM echanismsofAnthraq uinonfromRheumpalm atumL. TANGDa xuan,TANZheng huai，LIANG Yuan yuan, CHENG LEi，HUANGLi Instit uteofChineseMateri aMedica,Chengdu610041, China； DaliCo liege, Yunnan 67100 0，China Abstract： 0 bjectiveTocomparet heefficiencyandmec hanismsofdiarrheai nducedbyanthraquin onandglycosidesofa nthraquinonefromRh eumPalmatumL. inmic e. MethodsThelapact iceffectsofanthraq uinonandglycosides ofanthraquinonefro mRheumPa1mat umL. we restudiedinthemice whichweregivenhigh concentrationofpow deredmilksolutiont oinducedsupernutri tion,andtheeffects ofthesedrugsonthea ctivityofpepsinwer emeasuredinvitro. T heeffectofmilkonth elapacticactionofa nthraquinonandglyc osidesofanthraquin onewereanalyzedusi ngthefactorialdesi gn.ResultsTheanthr aquinoneofRheumpal matumL. 500 ， lOOOrng/ kgsignificantlyinc reasedtheremnantin  thegastricandsmall intestineinmice,an dtheglycosidesofanthraquinonefromRhe umPalmatumL. 500 10 OOmg/kgalsosignifi cantlyincreasedrem nantinsmall intestirugscouldincreaset rotEIninthegutinmi factoricaldesignsh uinoneorglycosides heumpalmatumL. werecreasetheremnantan nantinsmall intesti rugscouldincreaset rotEIninthegutinmi factoricaldesignsh uinoneorglycosides heumpalmatumL. were creasetheremnantan ioninstomach,small astrointestinaltra trationofCl-insmal 1 intestineandcolon trationofK+incolon adthesimilareffect actionofanthraquin L. Invitro,anthraqu fanthraquinonefrom noactionontheactiv s