《四角蛤蜊提取物抗氧化活性研究》-毕业论文.docVIP

精品 四角蛤蜊提取物抗氧化活性研究 摘 要：目的：对四角蛤蜊抗氧化活性进行评价研究。方法：采用水提醇沉的工艺路线分别得到四角蛤蜊总提液(MV)、醇沉沉淀(MP)及醇沉上清液(MS)。采用背部皮下注射D-半乳糖建立小鼠衰老模型，评价MV、MP及MS对模型小鼠肝脏与血清中SOD、MDA、GSH-PX、CAT各项指标的影响。以不同极性溶剂对MS萃取，得到石油醚、乙酸乙酯、正丁醇与水部位，评价各部位清除DPPH自由基、清除超氧自由基、螯合和还原铁的能力。结果：给予MV、MP、MS的小鼠肝组织SOD、GSH-PX、CAT显著升高(P＜0.05)，MDA显著降低(P＜0.05)，血清的SOD、GSH-PX显著升高(P＜0.05)，MDA显著降低(P＜0.05)。MS萃取水部位对DPPH自由基清除、超氧自由基清除和还原能力最强；MS萃取乙酸乙酯部位螯合能力最强。结论：四角蛤蜊具有较好的抗氧化活性，其活性较强部位是MS萃取水部位。 关键词：四角蛤蜊；提取液；抗氧化 Antioxidative activity of extracts from Mactra Veneriformis JI Jing, LIU Rui, WANG Ling-chong, CHEN Shi-yong, WU Hao?, WU Qiu-hui (Nanjing University of Chinese Medicine, Jiangsu Province center for research develop of marine drug, Nanjing 210046, China) Abstract：Objective: Tracking the activity though preliminary separation, we can figure out the chief substances of Mactra Veneriformis in antioxidant. Methods: According to the process line of water extraction and alcohol precipitation, total clam extract (MV), alcohol precipitation (MP) as well as supernatant (MS) have been got. Except the control group, all the other groups were subjected to subcutaneous injection of D-galactose solution. Mice were treated with three samples once a day for the creation of them low and high-dose groups. The activities of SOD and GSH-PX, CAT and the content of MDA in serum and liver were determined after a trial period of 6 weeks. Four sections were separated form competitive part and verified the activity of scavenging free radical, chelating ability of ion and reducing power. Results: The activities of SOD, GSH-PX and CAT exhibited a significant increase(P＜0.05), the activities of MDA also decrease in liver (P＜0.01) due to Mactra Veneriformis treatment. The activity of GSH-PX and SOD in serum exhibited an obvious increase(P＜0.05); meanwhile, an obvious decrease of MDA activity in serum was also observed(P＜0.05). Furthermore, extracts of MS exhibited the scavenging efficacy of O2- and DPPH，in which the water extracts showed the significant effect,