猪传染性胃肠炎病毒RT-LAMP检测方法的建立与初步应用.pdfVIP

CONTENTS CONTENTS Abstract．…………，……………………………………………………………………………………………………………，I Abstractin English……………………………………………………………………………．IⅡ 1Instruction……………………………………………．……………………………………………………………………1 ofTGE……………………………………………………………………………………………………．·1 1．1Summry 1．1．IParticlestructureofTGEV…………………………………………………………………………………2 1．1．2The celllinesofTGEV．．……．．．．………………．．．…．．．…．．．．．．．．．…．…．．．………………．2 susceptible 1．2Traditional methodsofTGEV…．…．．．…………．．…．．．．．…．．，，．．．…．．．．．…．．……………．．．．．．．2 diagnostic selectofinterest 1．2．1TGEVstructuralandthe gene．．．．．．．．…………………．．．…．．．2 major protein 1．2．2Virusisolationandidentification………………………………………………………………………．．3 all 1．2．3Detectedelectron by microscope………………………．………．．．……………·-·…·…………-····4 1．2．4 1．2．5ELISA．………．．…．…．．．．．．…．…………．．．…．．…．．．……．…．．…．．………………．．．．．．…．…．．．．……．，．．．．．．．．．．4 1．2．6Nucleicacid probe．…………………．…．．．…．．．．．．．…．…．…．．．…………．．…．．．．．．………．．…．．……．-．．…·5 1．2．7Virusneutralization test…………………．．…．．．．，．．．．……．．…………．．，．一．．．．…．．…．．．．…．．…，．．………5 1．2．8RT．PCR…………………………………………………………………………………………………．………．5 isothermal 1．3Reverse loop—mediatedamplification(RT—LAMP)。．．．．．．．．…………。．．5 transcripti011 1．3．1Primer ofRT-LAMP………………．．…．．…·．．．…．．…·····…．…………………-·····6 design principle 1．3．2 ofRT．LAMP……………………………………………………………………7 Amplificationprocess 1．3．3ThefeaturesofRT．LAMP……………………．．…．…．…．．．…．．．…．．．．．．，．．．．……………．．．…．．…．…．．．8 1．3．4 ofRT．LAMP．．．…………．……．，．．．．．．．．．．．．，，．…．．．．．．．．，．………．………．．．．．…．-，．·····．一9 Theproblems 1