PEG沉淀法_病毒提纯方法.docVIP

专业资料 精心整理 1. Viruses such as baculovirus can be concentrated by polyethylene glycol precipitation.2. Adjust the pH of the virus-containing supernatant to 7.2-7.5. Polyethylene glycol (PEG, MW 6,000) is added to a final concentration of 8% (w/v).3. Stir at 4℃for 2 hours.4. Centrifuge at 10,000 x g for 2 hours.5. Resuspend the pellet in a small volume of appropriate buffer ( e.g., RNase-free ddH2 for virus RNA prep). 沉淀法-病毒提纯方法主要是从稀的悬浮液中浓缩病毒，有中性盐沉淀法、聚乙二醇(PEG)沉淀法、