一株发酵纤维素产丁酸菌及其代谢动力学特性.PDF

中国环境科学 2016,36(1) ：232~237 China Environmental Science 一株发酵纤维素产丁酸菌及其代谢动力学特性 迟 雪 1,李建政 1*,艾斌凌 2 (1.哈尔滨工业大学,城市水资源与水环境国家重点实验室,黑龙江 哈尔滨 150090 ； 2. 中国热带农业科学院海口实验站,海南 海口 570102) 摘要：为开发降解纤维素产丁酸菌的种子资源,从牛粪、猪粪堆肥、玉米地土壤和腐木混合物的富集样品中分离得到一株厌氧降解纤维素 产丁酸菌.该菌株细胞呈杆状,长 7.1~9.1µm,直径 1.2µm 左右,经鉴定为丁酸梭菌(Clostridium butyricum),命名为 C. Butyricum DCB.在 35℃条 件下,菌株 DCB 在纤维二糖液体培养基中的最大比生长速率为 0.6536h- 1,世代时间为 1.06h,纤维二糖降解速率为 0.1g/(L·h),丁酸生成速率为 0.06g/(L·h).该菌株利用纤维素发酵产丁酸的转化率高达 0.23g/g,具有良好的开发前景. 关键词：纤维素；丁酸；丁酸梭菌；转化率；代谢动力学 中图分类号：X172,X382 文献标识码：A 文章编号：1000-6923(2016)01-0232-06 1 1* Butyric production and metabolic kinetics of a novel cellulose degrading strain. CHI Xue , LI Jian-zheng , AI Bin-ling2 ( 1.State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China；2.Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Haikou 570102, China). China Environmental Science, 2016,36(1)：232~237 Abstract ：A novel anaerobic strain, which could digest cellulose during butyric acid production, was isolated from an enrichment culture of cow dung, pig manure, corn soil and rotten wood. The strain was a bacillus with a length of 7.1~9.1 µm and a diameter of about 1.2 µm. It belonged to Clostridium butyricum and named C. Butyricum DCB. Strain DCB had a specific growth rate of 0.6536h- 1 in cellobiose medium at 35 ℃. In addition to this, the generation time, degradation rate and butyric acid production rate of Strain DCB were 1.06h, 0.1g/(L·h) and 0.06g/(L·h), respectively. In cellulose medium at 35℃, the highest butyric acid convertion ratio was 0.23g/g. Key words ：cellulose ；butyric acid ；Clostridium butyricum；conversion rat