《大叶醉鱼草化学成分的提取、分离、纯化研究》-毕业论文（设计）.docVIP

大叶醉鱼草化学成分提取、分离、纯化研究 摘 要 大叶醉鱼草(Buddleia davidii）属于马钱科醉鱼草属（Buddleja L．）。醉鱼草属植物中有很多种类由于形态优美、叶片对称和芳香宜人，因此是极具开发价值的园林植物，可用于园林绿化、室内观赏。现在许多国家已将醉鱼草属观赏植物运用于商业，利用高科技培育了许多比野生种更美的栽培品种，其中大叶醉鱼草是研究开发利用最为深入的种类。它主要分布在长江流域，在贵州它主要分布在贵阳、遵义、六盘水、毕节和安顺等地。在医药上大叶醉鱼草主治风湿性关节疼痛、跌打损伤和流行性感冒。有些地方甚至用它的根来治疗皮肤病和烧烫伤。现在已有大叶醉鱼草化学成分的相关报道，通过这些报道我们发现该植物中主要含有黄酮类、苯乙醇苷类、木质素、萜类等化合物，这些结果为研究开发利用大叶醉鱼草，为大叶醉鱼草生物活性研究提供了相关的理论依据。 本论文对其化学成分进行了较为系统的研究，运用硅胶柱层析、凝胶柱层析、聚酰胺柱层析、大孔吸附树脂、反相硅胶等分离手段进行分离，获得单体化合物。并综合利用IR、UV、MS、1H-NMR、13C-NMR、1H -1H COSY等现代波谱方法分离、鉴定得到了两个化合物，分别为：木樨草素和金合欢素-7-O-芸香糖苷。 关键词：大叶醉鱼草，化学成分，木樨草素, 金合欢素-7-O-芸香糖苷 Abstract Buddleia davidii is one of Buddleja L..Because of many kinds of Genus Buddleja have beautiful shape, symmetry leaves and fragrant, they are of great value garden plants, which can be used for landscaping and indoor ornamental. Now Buddleja Plants have been used in business in many countries. People use high-tech training a lot of species more beautiful than the wild cultivars, which the Buddleia davidii is the most in-depth research and development of the type of use. It is mainly distributed in the Yangtze River basin, and distributed in Guiyang, Zunyi, Liupanshui, Bijie and Anshun and other places in Guizhou. In medicine Buddleia davidii are used to treat rheumatic joint pain, bruises, and influenza. Some places even use it to treat skin diseases and burns roots. There have been reported the chemical constituents of Buddleia davidii. Through these reports we found that this plant mainly contains flavonoids, phenyl glycosides, lignin, terpenoids and other compounds, These results for studying, developing and using Buddleia davidii, and for studying the bioactivities of Buddleia davidii provides relevant theoretical basis. In the study, using Silica gel column chromatography, gel column chromatography, poly- amide column chromatography, macroporous resin, reverse phase separation of silica and other means of separation, single compounds were obtained. And com