Mas基因沉默对血管紧张素(17)拮抗血管紧张素Ⅱ诱导的肾间质成纤维细胞活化的影响.doc

Mas基因沉默对血管紧张素(17)拮抗血管紧张素Ⅱ诱导的肾间质成纤维细胞活化的影响.doc

- --- interstitial fibroblast activation, we use Mas SiRNA that selected already to transient transfect the NRK-49F. After cultured for 48h, cells is divided into 6 groups: ① normal control group (NG): the cells are maintained in the DMEM-F12 medium supplemented with 10% FBS and 1% penicillin-streptomycin. ②AngⅡ group: the cells are maintained in the DMEM-F12 medium supplemented with 10% FBS and the angiotensinⅡ that the final concentration was 10-6mol/L. ③Ang-(1-7) group: the cells are maintained in the DMEM-F12 medium supplemented with 10% FBS and the angiotensin-(1-7) that the final concen

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