低频rTMS对免疫介导的MND豚鼠的治疗作用及其机制分析-神经病学专业论文.docx

华中科技大学硕士学位论文 华 中 科 技 大 学 硕 士 学 位 论 文 PAGE 4 PAGE 4 Therapeutic effects and mechanisms of low frequency repetitive transcranial magnetic stimulation on guinea pigs with immune-mediated motor neuron disease Department of neurology，Union Hospital, Tongji Medical college， Huazhong University of Science and Technology Master candidate: Qi Peng Tutor: Prof. Mei Yuanwu Abstract PartⅠ：Effect of low frequency repetitive transcranial magnetic stimulation on survival time and motor function of guinea pigs with immune-mediated motor neuron disease Objective: To investigate the effect of low frequency repetitive transcranial magnetic stimulation(rTMS) on survival time and motor function of guinea pigs with immune-mediated motor neuron disease (MND) and to study the therapeutic action of low frequency rTMS on amyotrophic lateral sclerosis（ALS）. Methods: Models of experimental autoimmune gray matter disease （EAGMD） were established by using fresh bovine spinal cord anterior horn homogenate to immune guinea pigs. Guinea pigs were divided into treatment group and sham treatment group at the second to fourth weeks after immunizing at the second time as the peak of the disease. The treatment group was given by rTMS at 1Hz five days a week for two weeks. The sham treatment group was given sham treatment five days a week for two weeks. The motor function between the two groups was assessed by the inclined plate test. The survival time was also observed and compared between them. Results: The degrees of the inclined plate test were greater in part of treatment group on the second day after treatment than before. However, there was no significant difference in the degrees of the inclined plate test in total treatment group before and after treatment（P＞0.05）. The degrees of the inclined plate test descended in treatment group on the ninth day after treatmen（t P＜0.05）. The degree of the inclined plate test in treatment group was greater than that of sham treatment one（P＜ 0.05）.But the su