电刺激干预对废用性肌萎缩大鼠线粒体生物合成和呼吸功能的影响-体育学专业论文.docx

电刺激干预对废用性肌萎缩大鼠线粒体生物合成和呼吸功能的影响 电刺激干预对废用性肌萎缩大鼠线粒体生物合成和呼吸功能的影响 万方数据 万方数据 ABSTRACT Objective：This article focuses on establishing the model of muscular atrophy of disuse and recovering by electrostimulation. We try to investigate the mitochondrial biogenesis and respiratory function in skeletal muscles of rat. Further more, we study the effects of electrostimulation on mitochondrial biogenesis and respiratory function in skeletal muscles of rat after the muscular atrophy of disuse occurred. Methods：The SD male rats were devided into four groups: contrl group(A), atrophy group(B), every other day-stimulate group(C), everyday-stimulate group(D). The rats in the last three groups were colligated by tailor-made mould. Meanwhile, the rats in the last two groups were stimulated by electricity for everyday and every other day. After three weeks training, we measured the body weight, wet weight/body weight, maximal strength, average strength. We tested mRNA of myosin heavy chain (I, IIa, IIx, IIb) by real-time PCR and the proteins of PGC-1α and COXIV by Western Blot. This study also refers to the content of R3, R4, RCR and H2O2 in skeletal muscles of rats. Result：After three weeks training, the body weight of rats decrease and group C decrease obviously. Wet weight/body weight, maximal strength and average strength are lower in group B and D, while it is not sharp in group B. There are lower MHC I mRNA and higher MHC IIa, IIx, IIb mRNA after atrophy, and no differences in the last three groups. Compared with group A. the protein of PGC-1α and COXIV are decrease. They are increase after stimulated by electricity. Rroup D is increase obviously. R3, R4, RCR is decrease in group B and increase in group C and D. There are more changes in group D for R3 and R4. There is the same level for RCR in the last three groups. The levels of H2O2 in group B and D are most of all, then group C is second, group A is last. Conclusion：1)The protein of PGC-1α，COXIV and RCR decrease after atrophy, i