丙交酯端羧基PLGA与壳聚糖接枝共聚材料的制备及初步评价学位论文.docVIP

丙交酯-端羧基PLGA与壳聚糖接枝共聚材料的制备及初步评价--学位论文 丙交酯-端羧基PLGA与壳聚糖接枝共聚材料的制备及初 步评价--学位论文 暨南大学硕士学位论文 丙交酯/端羧基 PLGA与壳聚糖接枝共聚材料的制备及初步评价 摘 要 壳聚糖与聚乳酸是具有良好生物相容性的高分子材料,被广泛用于生物医学工程领 域,特别是作为组织工程支架。本文分别研究了丙交酯及端羧基聚乳酸/聚乙醇酸(PLGA) 与改性壳聚糖接枝聚合物的制备与性能。以壳聚糖为原料,经过改性制备了硅烷基(TMS) 保护壳聚糖,与丙交酯/端羧基 PLGA接枝聚合制备共聚物。采用傅立叶变换红外光谱、核 磁共振氢谱证实了共聚物的形成。热分析测试表明,共聚物具有不同于聚乳酸的热特性。 接触角测试表明共聚物具有较好的亲水性。降解实验考察了壳聚糖与 PLGA接枝共聚物膜 的降解行为。由于 PLGA有相对较强的疏水性,壳聚糖与 PLGA接枝共聚物的降解率随着 PLGA含量增加而下降,降解率可以通过 PLGA含量调节。本实验选用成纤维 细胞,采用 体外细胞培养法及 MTT测试评价壳聚糖与 PLGA接枝共聚物的生物相容性。 SEM 分析结 果表明细胞可以在共聚物膜上较好地粘附、增殖。MTT试验证明,生长在共 聚物膜上的细 胞具有良好的增殖趋势,表明了壳聚糖与 PLGA接枝共聚物对成纤维细胞具 有良好的生物 相容性。壳聚糖与 PLGA接枝共聚物具有优良的性能及生物相容性,可望作 为新型组织工 程支架材料。 关键词:壳聚糖;聚乳酸;接枝共聚;组织工程支架;生物相容性I暨南大学硕 士学位论文 丙交酯/端羧基 PLGA与壳聚糖接枝共聚材料的制备及初步评价 ABSTRACT Both chitosan and polylactide are good biocompatible polymers which are widely used in biomedical engineering, especially as scaffolds in tissue engineering. The copolymers of lactide/PLGA-COOH and chitosan were prepared and characterized in this paper. Firstly, the trimethylsilyl-protected chitosan was prepared using chitosan as the raw material, the copolymers were synthesized by the graft copolymerization of trimethylsilyl-protected chitosan with lactide or PLGA-COOH. The structures of copolymer products were characterized with 1 FT-IR or H NMR analysis. In addition, results of DSC testing illustrated that the thermal properties of copolymers were different from single PLA and the modified chitosan; the contact angle test showed the copolymers were more hydrophilicity than single PLA. The hydrolytic degradation properties of CTS-PLGA membranes were also investigated in vitro. The degradation rate decreased with an increase of PLGA content in copolymers and could be controlled by changing PLGA content. The biocompatibility of CTS-PLGA copolymers were investigated by in vitro cell culture and mitochondrial activity assay MTT. SEM images provided evidences of good adhesion,growth on CTS-PLGA membranes of 3T3 cells. MTT assay demo