高通量测序技术及原理介绍教案.ppt

Solexa 捕获技术 Paired-end vs. Mate-Pair SOLiD? System Mate-Paired Library Preparation Solexa Mate Pair Library I Solexa Mate Pair Library II 454 Long Span Paired-End I 四种关键酶 （荧光基团漂白） genomic DNA sheared DNA EcoP15I CAP linkers ligated on to sheared, methylated DNA digestion FDV-RDV ligated library molecules biotinylated internal adaptors with 25-27bp tags from genomic DNA sheared, methylated DNA shearing end repair methylation ligation circularization circularized DNA with biotinylated internal adaptors ligation 454 Long Span Paired-End II Digestion of non-circular DNA 降解线状DNA（ATP depende