基于近红外光谱法的鱼粉快速判别-食品科学.PDF

260 2015, Vol.36, No.24 食品科学 ※安全检测 基于近红外光谱法的鱼粉快速判别 宋 涛，宋 军，刘耀敏，米学林，饶瑾瑜，赵 艳，范秀丽 （通威股份有限公司，水产畜禽营养与健康养殖农业部重点实验室，四川成都 610041 ） 摘  要：基于近红外漫反射光谱分析技术对市场上常见的淡水鱼粉、进口鱼粉和国产鱼粉3 类商品化的鱼粉样品进 行自动化判别实验。通过分析鱼粉样品光谱之间的差异，采用主成分分析法建立鱼粉种类的定性判别的分类模型， 光谱范围为波长1 100～2 498 nm，交互定标决定系数为0.913 5，交互定标标准误差为0.133 8。通过对验证样品的分 析，建立的判别模型预判准确率达到84.6%，外部验证准确率达到100%。结果表明，近红外光谱技术结合化学计量 学法可以作为一种快速、无损、可靠的方法用于鱼粉种类的判别。 关键词：近红外光谱；鱼粉；判别；种类；主成分分析 Rapid Discrimination of Different Fishmeals with Near-Infrared Spectroscopy SONG Tao, SONG Jun, LIU Yaomin, MI Xuelin, RAO Jinyu, ZHAO Yan, FAN Xiuli (Key Laboratory of Aquatic, Livestock, Poultry Nutrition and Healthy Culturing, Ministry of Agriculture, Tongwei Co. Ltd., Chengdu 610041, China) Abstract: A method was established for the automatic discrimination of three varieties of fishmeal by means of near infrared ferences ferent spectroscopy(NIRS).Throughanalysisofthe spectraldif offishmealsamples,adiscriminationmodelfordif typesoffishmealwasdevelopedbyusingtprincipal componentanalysis(PCA).Thespectrawere scannedfrom 1100nm to 2 498 nm. The 1 minus variance ratio (1-VR) was 0.913 5 and the standard error of cross validation (SECV) was 0.133 8. The accuracy rates of discriminate for calibration and external validation were 84.6% and 100%, respectively. The results of the study indicate that NIRS combined with chemometrics is rapid, nondestructive, reliable and suitable for the discrimination of three varieties of fishmeal. Key words: near-infrared spectroscopy; fishmeal; discrimination; varieties; princip