烟草中植物甾醇的形态及分布-烟草科技.PDF

/TobaccoScienceTechnology /TobaccoChemistry 2009 1( 258 ) 1 2 3 1 1 1 , , , , , 1.中国科学技术大学烟草与健康研究中心,合肥市徽州大道 1129 号 230052 2.红河烟草 (集团)有限责任公 技术中心,云南省弥勒县桃园路 50 号 652300 3.云南烟草科学研究院, 昆明市高新区科医路 41 号 650 106 :植物甾醇;气相色谱;烟草;皂化 :采用浓盐酸水解、KOH乙醇溶液皂化、正己烷萃取、双 (三甲基硅烷基 )三氟乙酰胺 (BSTFA)衍生 化和气相色谱法测定了不同种类、不同品种、不同产地、不同部位烟叶样品中游离态和结合态菜油甾醇、 豆甾醇和 β-谷甾醇的含量。结果表明:①各甾醇的检测限在 0.10 ～0.35μg/mL范围, 相对标准偏差 2.3%～3.3%, 回收率 87%～99%;②烟叶中植物甾醇的总含量与其类型、产地、部位等有非常大的关 系;③烟叶中植物甾醇的种类分布也受品种的影响, 但部位、产地的影响不大;④烟叶中酯态甾醇 (SE) 和糖苷态植物甾醇(SG+ASG)的含量可能分别与其高级脂肪酸和单糖含量正相关。 :TS411.1 :A:1002 -0861 (2009 )01-0029-09 StateandDistributionofPhytosterolsinTobacco LIUSHAO-MIN(1 ), ZHOUGUI-YUAN(2 ), FANGLI(3 ), TONGHONG-WU(1), YANXIANG-YANG (1), andLIUWEN-HUI(1) 1.ResearchCenterofTobaccoandHealth, UniversityofScienceandTechnologyofChin,a Hefei 230052, China 2.TechnologyCente,rHongheTobacco(Group)Co., Ltd., Mile652300, Yunnan, China 3.YunnanAcademyofTobaccoScienc,eKunming650106, China Keyword:sPhytostero;lGC;Tobacco;Saponification Abstrac:tThecontentsoffreeandcombinedcampestero,lstigmasterolandβ-sitosterolintobaccoleavesof differenttype,svarietie,sgrowingareasandstalkpositionsweredeterminedbygaschromatographyvia hydrolysisbyconcentratedhydrochloricacid, saponificationbyanalcoholicKOHsolution, extractionwith hexan,eandderivationwithbis(trmiethylsilyl)trifluoroacetamide(BSTFA).Theresultsshowedthatthe lmiitsofdetection(LODs)ofthesterolsvariedfrom0.10 to0.35 μg/mLandtheirrecoveriesrangedfrom 87% to99% withtherelativestandarddeviations(RSDs)of2.3% to3.3%.Thetotalphytosterolcontentin tobaccoleafwasgreatlyaffectedbytobaccotyp,estalkpositionandgrowingarea.Thedistributionofdifferent kindsofphytosterolintobaccoleafwasalsoinfluencedbytobaccovariety, whiletheeffectsofstalkposition andgrowingareawerenotobvious.Thecontentsofsterylester(SE), sterylglycosideandacylatedsteryl glycoside(SG+ ASG)mightbepositivelycorrelatedwith