常用荧光染料的激发发射波长.pdfVIP

激发波长 发射波长 荧光素名称 备注 (nm) (nm) 1,5 IAEDANS 336 490 1,8-ANS 372 480 4-Methylumbelliferone 385 502 5-carboxy-2,7-dichlorofluorescein 504 529 5-Carboxyfluorescein (5-FAM) 492 518 5-Carboxynapthofluorescein (pH 10) 512/598 563/668 Ratio Dye, pH 5-Carboxytetramethylrhodamine (5-TAMRA) 542 568 5-FAM (5-Carboxyfluorescein) 492 518 5-HAT (Hydroxy Tryptamine) 370-415 520-540 5-Hydroxy Tryptamine (HAT) 370-415 520-540 578 604 5-ROX (carboxy-X-rhodamine) 567 591 548 552 5-TAMRA (5-Carboxytetramethylrhodamine) 542 568 6-Carboxyrhodamine 6G 518 543 6-CR 6G 518 543 6-JOE 520 548 7-Amino-4-methylcoumarin 351 430 7-Aminoactinomycin D (7-AAD) 546 647 7-Hydroxy-4-methylcoumarin 360 449, 455 9-Amino-6-chloro-2-methoxyacridine 412, 430 471, 474 ABQ 344 445 Acid Fuchsin 540 630 ACMA (9-Amino-6-chloro-2- 412, 430 471, 474 methoxyacridine) Acridine Orange + DNA 502 526 Acridine Orange + RNA 460 650 Acridine Orange, both DNA RNA 440-480 520-650 Acridine Red