高铜和高锌对饲料中益生菌活性的影响-宝来利来.PDF

26 中国饲料 2016 年第 19 期 DOI ：10.15906/j.cnki.cn11-2975/s 高铜和高锌对饲料中益生菌活性的影响 汪祥燕* ， 辛国芹， 赵 影， 董佩佩， 刘秀侠， 徐海燕， 谷 巍 （山东宝来利来生物工程股份有限公司，山东泰安 271000 ） 要 益生菌活性的影响，从而筛 摘 试验研究了饲料贮存过程及饲料中添加高水平铜和锌对本实验室保藏的 株 [ ] 9 选出活性高的益生菌，并以带毒平板法测定益生菌株对铜、锌的最大耐受浓度。 将 9 株益生菌分别制成单一菌制剂 添加到空白、高铜、高锌的仔猪料中，分别贮存 0 d 。 结果表明：无论在饲料贮存过程中还是添加高铜高锌后， 株芽 6 2 孢杆菌的活菌数均无显著变化。 3 株酵母菌对高铜、高锌的耐受性均较差。 4 株乳酸菌在贮存过程中活菌数有不同程 度降低，且对高铜高锌有不同耐受性，戊糖片球菌、植物乳杆菌与干酪乳杆菌、鼠李糖乳杆菌相比，对高铜、高锌抗逆 性相对较强，但活性不如芽孢杆菌。 键词 生菌；贮存；高铜；高锌；活性 [关 ] 益 [ 中图分类号] S816.7 [文献标识码] A [文章编号] 1004-3314（2016 ）19-0026-04 [Abstract] The experiment was conducted to study the effects of feed storing process and high level of copper and zinc on activity of nine strains of probiotics ， screen the high active probiotics and to determine strains maximum con- to centration of copper and zinc.Nine strains of probiotics were fermented and made into mono-cultures respectively.Then ， they were mixed in blank high level of copper and high level of zinc piglet feed and stored for sixty days respectively.The results showed that the two strains of Bacillus always maintained viable count both in the feed storing process or contain- ， ing high copper and zinc feed.On the contrary the tolerance of three strains of yeast to high level of copper and zinc was ，