不同竹种竹叶提取物制备及其抗氧化活性比较研究.PDFVIP

安徽农业大学学报, 2012, 39(4): 540-544 Journal of Anhui Agricultural University 网络出版时间：2012-7-4 17:47:17 [URL] /kcms/detail/34.1162.S1747.018.html 不同竹种竹叶提取物制备及其抗氧化活性比较研究 * 王 慧，岳永德 ，郭雪峰，汤 锋，王 进，姚 曦，孙 嘏 ( 国际竹藤中心，国家林业局竹藤科学与技术重点开放实验室，北京 100102) 摘 要：以毛竹、苦竹、香竹、箬竹、粉单竹、青杆竹和青皮竹7 个竹种竹叶为材料，采用热回流法提取，大 孔树脂法分离纯化，对得到竹叶提取物测定其得率和主要黄酮成分的含量，进而探讨竹叶提取物的抗氧化活性，为 竹叶提取物在食品和化妆品领域的应用提供科学依据。结果表明，苦竹叶提取物得率最高，为 3.60% ～3.70%；香 竹叶提取物中所含的荭草苷和异荭草苷量最高，为2.523 0% ～2.532 6% ，苦竹叶提取物中所含的牡荆苷量最高，为 0.887 0% ～0.889 6% ，青杆竹叶提取物中所含的异牡荆苷量最高，为2.223 1% ～2.226 1%；竹叶提取物具有较强的 自由基清除作用；香竹表现的DPPH 自由基清除能力最强，IC50 值为30.50 mg·L-1 ；箬竹的超氧阴离子和羟自由基 清除效果最强，IC50 值分别为339.32 mg·L-1 和324.87 mg·L-1 。 关键词：竹叶提取物；抗氧化活性；IC50 值 中图分类号：Q946.8 文献标识码：A 文章编号：1672−352X (2012)04−0540−05 Preparation and comparative analysis of antioxidant activity of bamboo leaf extracts from different varieties WANG Hui, YUE Yong-de, GUO Xue-feng, TANG Feng, WANG Jin, YAO Xi, SUN Jia (Centre for Bamboo and Rattan, State Forestry Administration Key Open Laboratory on Bamboo and Rattan Science and Technology, Beijing 100102) Abstract: Phyllostachys heterocycla, Pleioblastus amaru, Bambusa tuldoides, Bambusa chungii, Bambusa textilis, Chimonocalamus Hsueh and Indocalamus tessellates were used to extract bamboo-leaf-flavonoids by the heat reflux method and purified by AB-8 macroporous absorption resin. The extractions were separated and struc- turally identified by high-performance liquid chromatography (HPLC). The bamboo leaf extracts were assayed based on DPPH radical scavenging capacity, hydroxyl radical scavenging capacity, superoxide anion free radical scavenging capacity, and reducing power with TBHQ (tert-butyl hydroquinone) as the positive control. The results sho