连锁关联分析化在疾病研究中的探讨.pptVIP

* * Illumina’s genotyping assay is called the GoldenGate Assay It is a high specificity extension and ligation assay that allows the simultaneous analysis of over 1500 loci in a single reaction and uses the UNIVERSAL Sentrix arrays by incorporating into the reaction products a unique 地址 sequence for each locus being interrogated that is matched to a specific bead type’s illumiCode One of the most important features of the assay is that it uses genomic DNA and not amplified DNA as the genotyping target For each locus being interrogated 3 specific oligos are used 2 allele specific oligos (ASOs