中国大陆地区丛枝菌根真菌菌种资源分离鉴定和形态学特征.PDFVIP

微生物学通报 Oct. 20, 2016, 43(10): 2154−2165 Microbiology China /wswxtbcn tongbao@ DOI: 10.13344/j.microbiol.china.150921 研究报告 中国大陆地区丛枝菌根真菌菌种资源的分离鉴定与 形态学特征 * 王幼珊 张淑彬 殷晓芳 刘建斌 武凤霞 ( 100097) 摘 要：【目的】分离收集保藏中国大陆各个地区不同生态环境的丛枝菌根真菌菌种资源，为丛 枝菌根的研究提供资源、奠定基础。【方法】以高粱为宿主植物，采用诱导培养、单孢培养和扩 繁培养分离土壤样品中的丛枝菌根真菌菌种并鉴定。【结果】从我国大陆的45 个地区50 余种宿 主植物根区土壤中分离到丛枝菌根真菌 135 株，隶属于23 个种；对各个菌株的形态特征进行了 描述。【结论】我国蕴藏着丰富的丛枝菌根真菌菌种资源，文中描述的菌种资源是目前从我国大 陆地区获得的种类和数量最多、覆盖范围最广的AM 真菌菌种资源。 关键词：丛枝菌根真菌，分离，保藏，土壤 Isolation and identification of arbuscular mycorrhizal fungi from Mainland China * WANG You-Shan ZHANG Shu-Bin YIN Xiao-Fang LIU Jian-Bin WU Feng-Xia (Institute of Plant Nutrition and Resource, Beijing Academy of Agriculture and Forest Science, Beijing 100097, China) Abstract: [Objective] The aim of this study is to isolate the arbuscular mycorrhizal fungi (AMF) from different ecological environments in China, and lay the foundation for the research of AMF. [Methods] Relying on sorghum as the host plant, AMF strains were isolated from the soil using the trap cultures, single species cultures and stimulation of sporulation technologies respectively, followed by the species identification. [Results] One hundred and thirty-five AMF strains belonging to 23 species were isolated from the rhizosphere soil of over 50 host plants in 45 regions of China, and their morph