红曲霉固态发酵及形态解析-发酵工程专业论文.docxVIP

天津科投大学硕t学位论文摘要 天津科投大学硕t学位论文 摘要 论文主要礤究了红魈霉骤态发酵和界嚣模拟培养过程中，含水爨、湿艘、 空气相对湿度对其生长及发酵生产Monacolin K和红曲色素的影响，并通过 诗簿秘委橡分援，量亿发酵：i遘程载形态傣息。 以液态发酵为对照，固态发酵Monacolin K产量为液态发酵的10倍以上， 其有明显优势。经萄霉固态发酵时，基黢含隶量为50％较为逶宣；32℃畜铡 于菌体的生长，25℃有利于Monacolin K产生，因此采用变温培养，即初期 32℃，到第9天时降溢蜀25℃培养，Monacolin K产率遂著撬高。 固态发酵过程十分复杂，因此采用界蕊模拟培养避行简化，界隧模拟培 养中生物量、产物量与温度、湿度的关系与固态发酵过程一致。根据菌体生 长呈现鹣除段蛙，建立了萤体生长动力学模型：({)抉速期； Xf【(1一辨)网力一ntM—w(2)减遮期：Xv=2pt“2+k (3)衰退期：XT=kXITIe一。 根据表面分形理论，定义了菌落面积、表饺体积和表面缎理熵，建立了 蕾落图像分析系统，分析发酵过程瀚菌俸澎态。 菌落形态翱颜色镶形态特征反映了菌体活性，与麓体生长、产物生成密 切相关。快速期用菌藩面积来衡量菌体活性，减速期与衰减期菌体活性采用 表摄棼织v表示，建立了形态动力学模型：(t)抉速增长期；S=／a”’(2) 减速期：V=2∥tm十k(3)寝退期：V=eb心‘。菌体活性在快速期表现为 蘩体魏雯汝，减速期与衰减麓刘表瑷为次级找|罄产兹豹生藏，因此耀蕊热活 性和菌体生长速率共同描述微生物的产物生成，建立了产物生成动力学模烈： 警叫矿～0警)。 关键词： 固态发酵 爨嚣模掇 红越霉 菌体形态 计算机解析 天津科技大学硕士学位论文ABSTRACT 天津科技大学硕士学位论文 ABSTRACT It were discussed that the humidity，moisture content and temperature affected the product of Monacolin K and pigment in the process of solid—state fermentation and interface simulation culture in the article．Witll computer aid the morphological analysis of Monaseus was studied and described quantitatively the life of Monascus in different phased． Contrasting to the submerged fermentation(SMF)，the product of Monacolin K on SSF is over 10 times that of Solid—state fermentation(SSF)．On the Monascus solid—state fermentation，50％of initial moisture content is optimum． Temperature affected Monascus on its growth and product of Monacolin K respectively．The growth of mycelia at 32。C is better than that of at 25℃．The results showed that Monacolin K was distinctly improved by means of changing temperaturefrom 32*C to 25℃onthe 9thday． Simplifying the culture of Monascus in order to research SSF feasibly,the simulated system of gas-solid interface Was proposed．The characteristics of the Monascus growth and the product of Monacolin K on the SMF was consistent with that ofon the SSF．Kinetic model was derived on the basis ofgrowth different phages．The model of mycelia growth Was：(1)accelerating period： XT-【(1一竹)∥]爿一一，爿一一(