红霉素和阿仑磷酸钠抑制磨损颗粒诱导小鼠骨溶解的实验研究外科学专业论文.docxVIP

显降低 .C， D， E 组的 BVF 与 B 组 有 显 著 增 加 , E 组间的 BVF 较 C， D 有 明 显 增高 C，D 组间的 BVF 无 统 计 学 意 义 。病 理 学 骨 溶 解 区 域 破 骨 细 胞 数 量 各 组 比较结果 和 BMD 的 结 果 类 似 。 结论 ：红 霉 素 ，阿 仑 膦 酸 钠 可 以 抑 制 磨 损 颗 粒 诱 发 的 骨 溶 解 。有 望 成 为 预 防 人 工 关 节 无 菌 性 松 动 的 药 物 。 关键词 ： 红 霉 素 ， 阿 仑 膦 酸 钠 ， 磨 损 颗 粒 ， 骨 溶 解 ， 人 工 关 节 An in vivo study of inhibitory effect of Erythromycin and Alendronate on Titanim particle induced osteolysis ABSTRACT Objective： In present study an in vivo Titanium particles induced mouse calvarial ostelysis model was used to investigate the inhbitoy effect of erythromycin and alendronate over Titanium particles ind uced osteolysis． Methods ： Forty Female eight-week-old BALB/C species mice were randomly divided into 5 groups.8 in each grop.Control group (A) received sham operation; Titanium particles grop (B)received 30 mg Titanium particles implantation onto the calvariae ; alendronate group (C) received 30 mg Titanium particles implantation and alendronate 0.3 mg/kgweekly ip injection;one unit erythromycin group (D) received 30 mg Titanium particles implantation and ip injection EM 2m g/kg/day on the next week; two unit erythromycin group (E) ip injection EM 2m g/kg/day on the next day of 30 mg Titanium particles implantation; two weeks later mice calvaviae were harvested .The EM and ALN efficacy was evaluated by Micro-CTand histology. Results ： Micro-CT analysis showed that bone mineral density (BMD)and bone volume percentage (BVF) were significantly higher in the EM,Aln treated group(CDE) compared to the Ti group(B),(p0.05);BMD and BVF were significantly higher in the Control group (A) compare d to the Ti group(B),(p0.05); histology analysis had a similar result with BMD. Conclusion： Erythromycin and Alendronate can effectively inibite Titanium particles induced in vivo calvarial osteolysis ． KEYWORDS： Erythromycin， Alendronate ，Wear debris，Osteolysis ， Artificial joint 符 号 说 明 编 号 全称 中文 Micro-CT Micro-Computed tomography 显微 CT M Macrophages 巨噬细胞 BMD Bone Mineral Density 骨密度