久效磷对斑马鱼dani学o rerio2世代繁殖的影响.docxVIP

颜色最深且较宽，蛋白含量最高，表明O．10mg 颜色最深且较宽，蛋白含量最高，表明O．10mg L4久效磷诱导雄性斑马鱼VTG 能力最强，对雄鱼的内分泌干扰效应最强。我们认为0．10mg Lo久效磷对繁殖 的抑制效应最强，可能与VTG的大量被诱导产生有关。 (4)久效磷对雄鱼生殖腺组织结构的影响表现为，精原细胞、精母细胞以 及小叶基膜的水肿。对精子进行超微结构观察发现，久效磷对精子的影响主要 表现为对膜系统的损伤，细胞膜、核膜和线粒体的外膜内嵴都普遍出现水肿、 断裂和溶解现象，部分细胞周围出现空泡，可能与胞内物质出现外溢有关。其 中，久效磷对精子细胞期和囊内精子期生殖细胞发育的影响最大，细胞核染色 质不能完全收缩甚至出现溶解。谢尔托立氏细胞数量增多且胞质内具有大量被 吞噬的坏死精子。 (5)久效磷对雌鱼卵子发育过程的影响表现为，在卵母细胞发育早期阶段， 久效磷暴露使卵母细胞的核质发生萎缩，核仁形态异常，在卵母细胞大生长期 (卵黄卵母细胞)主要表现在卵黄发生溶解，仞生卵黄数量减少，次生卵黄发生 凝集，细胞核膜水肿部分溶解，与卵黄或细胞质出现互溶现象。超微结构也观 察到卵母细胞初生卵黄和次生卵黄发的溶解和萎缩，同时还观察到暴露早期卵 膜出现的外放射带局部断裂和内放射带结构溶解变薄的现象，随暴露时间延长 O．10mg L。暴露组卵膜出现外放射带局部融合和内放射带增厚的现象，可能是 卵子的细胞保护机制被激活，但卵膜厚度的这种变化影响了F。繁殖力，表明卵 子的卵膜结构变化情况可能与繁殖力的变化相关。 关键词：斑马鱼，久效磷，繁殖，卵黄原蛋白，2世代 H The The effects of monocrotophos on reproduction of zebrafish(Danio rerio)in two generations Abstract The environmental risk of organophosphorus pesticides(OP)increases rapidly with the improvement of the diversity and production of OPs．It has caused many environmental problems,such as the reproduction impairment，gonads alteration，the population decline,and so on。Monoerotophos(Mcp)，one of organophosphorons pesticides、vitll high water solubility and environmental estrogen activity showed potential impact on human health and aquatic organism．So in this study,a full life cycle chronic toxicity experiment was performed by exposing zebrafish(Danio rerio) to O．01、O．10andl．00mgLlMCPfortwo generation．Reproductiveparameters such as the total number of eggs，the number of eggs in once spawns，the total number of spawning，the fertility and hatch in F0(adult，exposed 28d)and F1(1SOd，exposed from fertility until adult to mating)were investigated．The induction of VTG‘a widely used indicator for estrogenic exposure in fish，in the FO male fish by 28d MCP exposure was determined by Western-blotting．In addition to the F0 gonads histopathological alterations were observed by light microscope and gametes by electron microscope，the reproduction effects of MCP on two generations of zebrafish were investigated as well．T