红景天苷预处理对大鼠肝脏缺血再灌注损伤的保护作用-外科学(普通外科学)专业论文.docxVIP

红景天苷预处理对大鼠肝脏缺血再灌注损伤的保护作用 中文摘要 目的 探讨在大鼠肝脏缺血-再灌注损伤中红景天苷预处理的保护作用。 方法 采用随机数字法将 SD 大鼠分为假手术组（18 只）、缺血-再灌注模型组（18 只）、红景天苷高浓度预处理组（18 只、24mg/kg）、红景天苷低浓度预处理组（18 只、6mg/kg）。模型建立成功后分别于 1h、6h、8h 各组处死 6 只大鼠留取血清和 肝组织，进行血清 ALT、AST 浓度的检测，肝组织热休克蛋白 70（HSP70）的表 达用免疫组化法测定，光镜下观察肝脏组织病理变化。 结果 实验结果显示各组血清中 AST，ALT 浓度于再灌注后 1 小时开始升高，6h 达最到高峰，8 小时已有所下降。各组与模型组进行比发现经红景天苷预处理的 肝组织病理损伤明显减轻，血清中 AST、ALT 浓度明显降低，HSP70 表达显著降 低（p＜0.05）。 结论 红景天苷预处理可以降低大鼠肝组织缺血再-灌注损伤过程中 HSP70 的表 达，其作用机制可能与其抗氧化、清除氧自由基等有关。 关键词 红景天苷;肝脏;缺血再灌注损伤;热休克蛋白 70 中图分类号 R657.3 文献标识码 A The protective effect of Salidroside preconditioning on hepatic ischemia-reperfusion injury in rats Zhang peng Abstract Objective To explore the protective effect of salidroside preconditioning on hepatic ischemia-reperfusion injury in rats. Method Seventy-two SD rats were randomly divided into four groups.Group A:pseudo-operation group;Group B:ischemia-reperfusion group;Group C:high doses of salidroside prevent group(24mg/kg);Group D:low doses of salidroside preventive group(6mg/kg).Serum and liver samples were collected at 1h,6h and 8h after the 4 models were established.Seurm alanine aminotransferase(ALT),aspartate aminotransf- erase(AST) were measured by an automatic biochemical analyzer,expression of Heat shock protein 70(HSP70) of the liver was detected by immunohistochemical staining and the pathological of liver were examined. Result In four groups,the concentrations of AST and ALT were increased at 1h,reached its highest level at 6h.Compared with I/R group in group of salidroside preconditioning,the pathological changes of the liver were ameliorated significantly,and the level of serum AST,ALT and the expression of HSP70 were significantly reduced(p0.05). Conclusion Salidroside preconditioning can protect liver from ischemia-reperfusion injury in rats by clearing Oxygen free radicals(OFR) and its antioxidant effect. Key Words: Salidroside;Liver;Ischemia-reperfusion injury;HSP70 前 言 众所周知，组织和器官要想获得充足氧和营养物质供应并排出代谢产物的基 本前提条件是具有良好的血液循环，造成细胞发生缺血性损伤的各种原因中组织