Lactobacillus pantheris TC102胞外多糖的提取纯化和功能特性研究.docxVIP

Lactobacilluspantheris 7(，102胞外多糖的提取纯化和功能特性研究 pantherTCPl02分泌的三种胞外多糖EPSl、EPS2和EPS3均具有多糖的特征 吸收峰，存在0c型吡喃糖环构型。GPC检测这三种EPS的重均分子量依次是 20292Da、22971Da和19301Da。EPSl主要由半乳糖、葡萄糖和甘露糖组成， 三者摩尔比葡萄糖：甘露糖：半乳糖为2．86：1．48：1。EPS2主要由半乳糖、葡萄 糖和甘露糖组成，三者摩尔比葡萄糖：半乳糖：甘露糖为1．26：1：1。EPS3主要 由半乳糖、葡萄糖和甘露糖组成，三者摩尔比半乳糖：葡萄糖：甘露糖为 1．80：1．58：1。 3．将EPSl、EPS2和EPS3作用于巨噬细胞Ana．1后发现，这三种胞外多糖 均能刺激巨噬细胞Ana．1增殖、产生NO、分泌TNF—Q和IL．6；用EPSl、EPS2 和EPS3刺激小鼠腹腔巨噬细胞后发现，这三种胞外多糖也能同时刺激腹腔巨噬 细胞产生NO，分泌TNF．Q和IL．6。结果表明，TCPl02分泌的这三种胞外多糖 对巨噬细胞具有激活作用。 4．j各EPSl、EPS2、EPS3作用于结肠癌HCT-1 16、结肠癌Caco．2、卵巢癌 A．2780、胃癌BGE．803细胞24、48、72h后发现，这三种胞外多糖对它们的生 长均有抑制效果，抑制率随糖浓度和时间的增加而增加，整体抑制率EPS3 EPS2EPSl。尤其是当EPS3浓度为50099／mL，抑制时间为72h时，对卵巢癌 A．2780的抑制效果最强，抑制率达到72％。 关键字：乳酸菌；胞外多糖；分离纯化；巨噬细胞激活；肿瘤抑制 万方数据  内蒙古大学硕士学位论文 EXTRACT I ON AND PUR I F I CAT I ON AND FUNCT I ONAL CHARACTER I ST I CS 0F EXOPOLYSACCHAR I DES FROM Lactobac i／／us pan ther is TCP 1 02 ABSTRACT Lactic acid bacteria (LAB) exopolysaccharides (EPSs) are capsular polysaccharide slime polysaccha-rides which secreted by LAB into the extracellular environment during growth，is the secondary metabolites of LAB．A number of studies have proved that as a kind of macromolecule material，LAB EPS has no cytotoxicities，can enhance the immune cell function，restrain the proliferation of cancer cell，antioxidant，antivirus，and other functions，having a broad application in the future． In this study,we isolated and purified the EPSs of Lactobacillus pantheris TCP 1 02 strain firstly．We further investigated the effects of purified EPSs on liferation’,ohagin macrophage andnd’nhibitionlnlaibltion ofOt cancer cell’ines．Thecell lines ● 1 ne resultsresults werewel?e as follows： 1．We optimized the factors affecting EPSs production of Lactobacillus pantheris TCP 1 02 which were culture medium and fermentation time，temperature， initial pH value and inoculation amount．The preferable culture conditions were inoculation amount of 4％，3 7。C，and pH 7 for 3 0h，cultured with MRS liquid medium．The EPS production was about 90