甘薯茎腐病菌的遗传多样性及致病力差异分析.PDF

植物保护学报Journal of Plant Protection ，2018 ，45 （6）：1227-1234 DOI ：10.13802/j.cnki.zwbhxb.2018.2017112 甘薯茎腐病菌的遗传多样性及致病力差异分析 * 黄立飞 陈景益 房伯平 罗忠霞 张雄坚 王章英 （广东省农业科学院作物研究所，广东省农作物遗传改良重点实验室，广州510640） 摘要：为了解不同地区甘薯茎腐病菌Dickeya dadantii 种群遗传多样性水平及致病力差异，采用重 复序列PCR 基因指纹（repetitive element palindromic PCR ，REP-PCR ）技术和薯片接种方法，对采自 广东省、广西壮族自治区和重庆市的6 个市区县的59株菌株进行分析。结果表明，5 对引物对59 株菌 株扩增出41 个清晰的条带，其中36 个为多态性条带，每对引物的扩增条带数在4~ 10之间，平均为 7.2 。在物种水平上，有效等位基因数、Nei ’s 基因多样性指数和Shannon 信息指数分别为1.4768 、 0.2801 和0.4186 ，其中湛江种群多样性最高，南宁种群多样性最低；当遗传相似系数为0.79 时，59 株 菌株可被划分为5 个类群，类群划分与菌株来源地间有一定的相关性。此外，不同地区病菌种群间 存在明显的致病力差异，其中合浦种群与湛江种群致病力最强，万州种群致病力较弱。表明甘薯茎 腐病菌种群具有丰富的遗传多样性，不同地区的病菌种群存在明显的遗传多样性与致病力差异。 关键词：甘薯茎腐病；达旦提狄克氏菌；遗传多样性；重复序列PCR 基因指纹；致病力差异 Pathogenic differentiation and genetic diversity of Dickeya dadantii causing bacterial stem and root rot of sweetpotato Huang Lifei Chen Jingyi Fang Boping* Luo Zhongxia Zhang Xiongjian Wang Zhangying （Guangdong Provincial Key Laboratory of Crops Genetics and Improvement, Crop Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China） Abstract: To understand the pathogenic differentiation and genetic diversity of Dickeya dadantii caus- ing bacterial stem and root rot of sweetpotato, 59 isolates collected from six different regions in Guang- dong Province, Guangxi Zhuang Autonomous Region, and Chongqing City were analyzed by using re- petitive-element PCR genomic fingerprinting (REP-PCR) techniques and chip inoculation methods. The results showed that 41 obvious bands were amplified, in which 36 bands were polymorphic. For each primer pair, four-ten alleles were detected with an average of 7.2. At the level of species, effective num- b