大孔树脂对红心萝卜花色苷的纯化.pdfVIP

※工艺技术 食品科学 2011, Vol. 32, No. 04 21 1 1 2 1 1 董周永 ，阿 嘎 ，赵国建 ，王笑丹 ，闫琳娜 (1.吉林大学生物与农业工程学院，吉林 长春 130022 ；2. 山西师范大学食品科学与工程系，山西 临汾 041000) 比较6 种大孔吸附树脂对红心萝卜花色苷的吸附解吸效果，研究 AB-8 型大孔树脂对红心萝卜花色苷的吸 附与解吸条件。结果表明，AB-8 型大孔树脂是纯化红心萝卜花色苷较适合的树脂类型；红心萝卜花色苷在 AB-8 型树脂上的吸附平衡时间 4h ，吸附最适温度20 ℃，花色苷溶液的最适吸附pH3.0 ，解吸时宜选用75% 乙醇溶液。 经纯化后的萝卜花色苷为紫黑色粉末，色价为 47.8 ，是纯化前的 12 倍。 红心萝卜；花色苷；纯化；大孔树脂 Purification of Anthocyanins from Red-core Radish by Macroporous Resin 1 1 2 1 1 DONG Zhou-yong ，A Ga ，ZHAO Guo-jian ，WANG Xiao-dan ，YAN Lin-na (1. College of Biological and Agricultural Engineering, Jilin University, Changchun 130022, China； 2. Department of Food Science and Engineering, Shanxi Normal University, Linfen 041000, China) Abstract ：The adsorption and desorption effects of 6 kinds of macroporous resins on anthocyanins from red-core radish were compared. The adsorption and desorption properties of resin AB-8 on anthocyanins from red-core radish were further investigated. Results indicated that the adsorption equilibrium time of anthocyanins on resin AB-8 was 4 h. The highest adsorption capacity of resin AB-8 towards anthocyanins from red-core radish was achieved under the conditions of pH 3 and 20 ℃. In addition, 75% ethanol could provide the best desorption effect on anthocyanins from resin AB-8. The purified anthocyanins obtained under the optimal conditions were purple dark powder with color value of 47.8, which exhibited 12-fold enhancement compared with the color value of anthocyanins before purification. Key words ：red-core radish ；anthocyanins ；p