北京萝卜萎蔫病病原鉴定及病原生长影响因子.PDFVIP

中国农学通报 2012,28(10): 116-120 Chinese Agricultural Science Bulletin 北京萝卜萎蔫病病原鉴定及病原生长影响因子 1 2 1 2 1 耿丽华 ，迟胜起 ，张 丽 ，宁利园 ，史 越 1 2 （国家蔬菜工程技术研究中心，北京100097；青岛农业大学农学与植保学院，山东青岛266109 ） 摘 要：2009 年北京郊区发生一种萝卜(Raphanus sativus L.)新病害，其主要症状表现是叶子黄化萎蔫，生 长受阻。针对该病的发生，作者采集典型发病植株进行病原菌的分离纯化，用传统的形态学方法和 rDNA-ITS 序列分析方法进行病原鉴定，并进一步对病原菌生长影响因子进行研究，以便为该病的进一 步深入研究、抗病种质资源的筛选和抗病品种的选育打下基础。研究认为该病由大丽轮枝菌 (Verticillium dahliae)侵染引起。该病原菌生长适宜温度为20~25℃，适宜pH 6~8，该病原菌能够很好地 利用多种氮源和碳源，氮源以硫酸铵最为适宜，碳源则已可溶性淀粉最适宜。这是首次报道由大丽轮枝 菌侵染引起的萝卜黄萎病在中国的发生。 关键词：萝卜；蔫萎病；病原；鉴定；影响因子；大丽轮枝菌 中图分类号：S436.31 文献标志码：A 论文编号：2011-2645 Identification of the Pathogen of Radish Wilt in Beijing Suburb and Factors Affecting Its Growth 1 2 1 2 1 Geng Lihua , Chi Shengqi , Zhang Li , Ning Liyuan , Shi Yue 1 ( National Engineering Research Center for Vegetables, Beijing 100097; 2 College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao Shandong 266109) Abstract: A new radish (Raphanus sativus L.) disease happened in Beijing suburb in 2009. The main symptom of the disease is that the infected radish grows stunt and it ’s leaves become yellow. We collected the typical diseased radish plants to isolate the pathogen, and identified the pathogen according to the morphology and the sequence of the internal transcribed spacer region of the ribosomal DNA (ITS), and also studied the major affecting factors of the pathogen in order to provide the basis to study controlling measures and to screen